Human Rubella Virus Antibody (IgG) ELISA Kit (Colorimetric)

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
serum, plasma
Standard Curve Range
Qualitative

Order Details

Human Rubella Virus Antibody (IgG) ELISA Kit (Colorimetric) Summary

Specificity
This assay has high sensitivity and excellent specificity for detection of human anti-rubella virus (RV) antibody (IgG). No significant cross-reactivity or interference between human anti-rubella virus (RV) antibody (IgG) and analogues was observed.
Standard Curve Range
Qualitative
Assay Type
Direct ELISA
Inter-Assay
CV%<15%
Intra-Assay
CV%<15%
Sample Volume
50-100ul
Kit Type
ELISA Kit (Colorimetric)

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8° C. Protect from light. Do not use past expiration date.

Kit Components

Components
  1. Wash Buffer (10 x concentrate)
  2. Substrate B
  3. Substrate A
  4. Sample Diluent
  5. Stop Solution
  6. Positive Control
  7. Adhesive Strip (For 96 wells)
  8. Coated assay plate
  9. Instruction manual
  10. HRP-conjugate
  11. Negative Control
  12. Cut-off Control

Notes

Please contact our Technical Services with any questions regarding species reactivity

Background

This assay employs the qualitative enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with antigen. Samples are pipetted into the wells with anti-human IgG conjugated Horseradish Peroxidase (HRP). Any antibodies specific for the antigen present will bind to the pre-coated antigen. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of human anti-rubella virus (RV) antibody (IgG) bound in the initial step. The color development is stopped and the intensity of the color is measured.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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