Mouse Rotavirus Antigen ELISA Kit (Colorimetric)

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications ELISA
Suitable Sample Type
Feces

Order Details

Mouse Rotavirus Antigen ELISA Kit (Colorimetric) Summary

Description
Assay Length: 1-5 hours
Specificity
This assay has high sensitivity and excellent specificity for detection of mouse rotavirus antigen. No significant cross-reactivity or interference between mouse rotavirus antigen and analogues was observed.
Assay Type
Sandwich ELISA
Inter-Assay
%CV < 15
Intra-Assay
%CV < 15
Sample Volume
50 - 100 uL
Kit Type
ELISA Kit (Colorimetric)

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Adhesive Strip (For 96 wells)
  2. Assay plate
  3. HRP-conjugate
  4. Instruction manual
  5. Negative Control
  6. Positive Control
  7. Stop Solution
  8. Substrate A
  9. Substrate B
  10. Wash Buffer

Background

This assay employs the qualitative enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to rotavirus antigen. Samples are pipetted into the wells with antibody conjugated Horseradish Peroxidase(HRP). Any rotavirus antigen present in samples will bind to the pre-coated specific antibody. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of mouse rotavirus antigen bound in the initial step. The color development is stopped and the intensity of the color is measured.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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FAQs for Rotavirus Antigen ELISA Kit (NBP2-60135). (Showing 1 - 1 of 1 FAQ).

  1. wondering what the difference is between your quantikine and duo set elisas?
    • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

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