Rotavirus Antibody - BSA Free

Product Discontinued

Rotavirus Antibody - BSA Free Summary

• Immunogen: Nebraska calf diarrhea virus
• Specificity: This is specific against ICPs & late structural (virion) antigens. Uninfected cell reactivity is negative against HEp-2 cells and WI-38 cells by indirect immunofluorescence.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity: Protein G purified

Applications/Dilutions

• Application Notes: Conjugation: Use at an assay dependent dilution. IF: Use at an assay dependent dilution. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Reactivity Notes

Cross-reacts (>90%) with Human rotaviruses.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.1% Sodium Azide
• Concentration: 4.0 mg/ml
• Purity: Protein G purified

Alternate Names for Rotavirus Antibody - BSA Free

• Major inner capsid protein VP6
• VP6

Background

Rotaviruses, members of the family Reoviridae, are a major cause of diarrhea in young mammals. Rotavirus infections also result in economic losses in agriculture due to diarrhea in calf, pig, sheep, and poultry rearing. Diarrhea (or scours) due to the rotavirus Nebraska Calf Diarrhea Virus can affect calves up to 30 days of age or older. Diarrhea begins 2 to 3 days after exposure. Diagnosis is by history, lesions (ulcers on the tongue, lips, and mouth) and laboratory tests. Mortality rates may be as high as 50 percent, depending on the secondary bacteria present. Human rotaviruses, the major etiological agents of severe infantile diarrhea worldwide, display very diverse and complex serotypic specificities. Rotaviruses are 70 nm, non enveloped viruses comprised of a triple layered protein capsid; Outer capsid proteins are VP4 and VP7, Inner capsid -VP6 and Core -VP2. The immunity acquired from exposure to rotavirus appears to be type specific following initial infection. Therefore, multiple serotypes of rotavirus mean multiple opportunities for infection. The combination of animal reservoirs for the virus and rotavirus gene reassortment provides the potential for dramatic genetic shifts (similar to influenza virus) which could give rise to altered host ranges and viral virulence.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.