Rib-P Ab ELISA Kit (Colorimetric) Summary
| Description |
Quality control test: Standard curve. Rib-P is an indirect solid phase ELISA for the quantitative measurement of IgG class autoantibodies against ribosomal P proteins in human serum or plasma. |
| Standard Curve Range |
12.5 to 200 U/mL |
| Sensitivity |
1.0 U/mL |
| Assay Type |
Indirect ELISA |
| Inter-Assay |
CV% < 3.8-6.4% |
| Intra-Assay |
CV% < 2.4-6.3% |
| Sample Volume |
10 uL |
| Kit Type |
ELISA Kit (Colorimetric) |
Applications/Dilutions
| Dilutions |
|
| Application Notes |
Useful for quantification. |
Packaging, Storage & Formulations
Kit Components
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Components
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- 1 vial, 15 ml: Enzyme conjugate solution (PBS, PROCLIN 300 <0.5% (v/v)), (light red) containing polyclonal rabbit anti-human IgG; labelled with horseradish peroxidase. Ready touse.
- 1 vial, 15 ml: Stop solution (contains acid). Ready to use.
- 1 vial, 15 ml: TMB substrate solution. Ready to use.
- 1 vial, 20 ml: Sample buffer (Tris, NaN3 <0,1% (w/w)), yellow, concentrate (5x)
- 1 vial, 20 ml: Wash solution (PBS, NaN3 <0,1% (w/w)), concentrate (50x).
- 2 vials, 1.5 ml each: Anti-Rib-P Controls in a serum/buffer matrix (PBS, BSA, NaN3 <0,1% (w/w)) positive (1) and negative (2), for the respective concentrations see the enclosedpackage insert. Ready to use.
- 6 vials, 1.5 ml each: Anti-Rib-P Calibrators (A-F) in a serum/buffer matrix (PBS, BSA, NaN3 <0.1% (w/w)) containing: IgG: 0; 12.5; 25; 50; 100; and 200 U/ml. Ready to use.
- Qty.1: Divisible microplate consisting of 12 modules of 8 wells coated with highly purifiedribosomal P proteins P0, P1 and P2 (Rib-P). Ready to use.
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Notes
This product is produced by and distributed for Abnova, a company based in Taiwan.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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FAQs for Rib-P Ab ELISA Kit (KA1110). (Showing 1 - 1 of 1 FAQ).
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What is the difference between the Quantikine and Duoset ELISA kits?
- Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.
