Rhodamine Antibody (11H10) - BSA Free

Western Blot: Rhodamine Antibody (11H10) [NBP1-69961] - Dot Blot of Mouse anti-Rhodamine Monoclonal Antibody. Antigen: Row 1 - Rhodamine Conjugated Streptavidin Row 2 - Streptavidin. Load: Column 1 - 100 ng Column 2 - 33.3 ng Column 3 - 11.1 ng Column 4 - 3.70 ng Column 5 - 1.23 ng.Primary antibody: Mouse anti-Rhodamine Monoclonal Antibody at 1:1,000 for 60 min at RT.Secondary antibody: HRP mouse secondary antibody at 1:40,000 for 30 min at RT.

Rhodamine Antibody (11H10) [NBP1-69961]

Dot Blot of Mouse anti-Rhodamine Monoclonal Antibody. Antigen: Row 1 - Rhodamine Conjugated Streptavidin. Row 2 - Streptavidin. Load: Column 1 - 100ng. Column 2 - 33.3ng. Column 3 - 11.1ng. Column 4 - 3.70ng. Column 5 - 1.23ng. Primary antibody: Mouse anti-Rhodamine at 1:1,000 for 60 min at RT. Secondary antibody: HRP mouse secondary antibody at 1:40,000 for 30 min at RT. Block for 1 HR at RT.

• Reactivity: All-NA
• Applications: WB, DB, ELISA, IHC
• Clone: 11H10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

Rhodamine Antibody (11H10) - BSA Free Summary

• Description: Rhodamine Monoclonal Antibody was protein A purified and reacts specifically with Rhodamine and its derivatives
  
  Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
• Immunogen: Rhodamine Antibody (11H10) was produced after repeated immunizations of balb/c mice with rhodamine conjugated KLH.
• Specificity: RHODAMINE Monoclonal Antibody was protein A purified and reacts specifically with Rhodamine and its derivatives. Rhodamine isomer 5 and isomer 6 are reactive as TAMRA, as well as TRITC conjugated proteins. No reaction is observed against Texas Red.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Dot Blot
  • ELISA 1:10000-1:30000
  • Immunohistochemistry 1:1000-1:10000
  • Western Blot 1:1000-1:10000
• Application Notes: This protein A purified monoclonal antibody against rhodamine reacts with most derivative molecules and has been tested for use in ELISA. Optimal concentration in other immunoassays should be determined by the researcher.

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Purity: Protein A purified

Alternate Names for Rhodamine Antibody (11H10) - BSA Free

• Rhodamine 6G
• Rhodamine B

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.