Human Rheumatoid Factor ELISA Kit (Colorimetric)

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Plasma, Serum
Standard Curve Range
0-500 U/mL
Sensitivity
1 U/mL

Order Details

Human Rheumatoid Factor ELISA Kit (Colorimetric) Summary

Standard Curve Range
0-500 U/mL
Sensitivity
1 U/mL
Assay Type
Indirect ELISA
Inter-Assay
CV% < 9.10%
Intra-Assay
CV% < 6.20%
Spike Recovery
80-113%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)

Applications/Dilutions

Application Notes
Rheumatoid factor IgA ELISA Kit is indirect solid phase enzyme immunoassay for the quantitative measurement of rheumatoid factor IgA in serum and plasma.

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Wash solution, containing Tris, detergent, preservative NaN3 0.09%, 50x conc.
  2. TMB substrate, containing 3,3’,5,5’- Tetramethylbenzidine, colorless. Ready to use.
  3. Stop solution, contains acid. Ready to use.
  4. Sample buffer P, containing PBS, BSA, detergent, preservative NaN3 0.09%, yellow,concentrate (5x).
  5. Calibrators A-E (0, 15, 50, 150, 500 U/mL), containing rheumatoid factor in aserum/buffer matrix (PBS, BSA, detergent, NaN3 0.09%), yellow. Ready to use.
  6. Control positive (1) and negative (2), containing rheumatoid factor in a serum/buffermatrix (PBS, BSA, detergent, NaN3 0.09%), yellow. Ready to use. The concentration isspecified on the certificate of analysis.
  7. Divisible microplate consisting of 12 modules of 8 wells each. Ready to use.
  8. Enzyme conjugate, light red, containing anti-human IgA antibodies, HRP labelled; PBS,BSA, detergent, preservative Proclin 300 0.05%, Ready to use.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Human Rheumatoid Factor ELISA Kit (Colorimetric)

  • RF

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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