Reg1A/B Antibody [Alexa Fluor™ Plus 647]

• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor Plus 647

Reg1A/B Antibody [Alexa Fluor™ Plus 647] Summary

• Specificity: Detects human Reg1A in direct ELISAs. In direct ELISAs, approximately 80% cross-reactivity with recombinant human (rh) Reg1B is observed, approximately 20% cross-reactivity with recombinant mouse Reg1, and less than 5% cross-reactivity with rhReg3A and recombinant rat Reg1A is observed. 100% cross-reactivity with rhReg1B in Western blot is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

Reg1A (Regenerating gene IA/ alpha ; also REG, ICRF, PSPS, PTP and Lithostathine-1 alpha ) is a secreted, variably glycosylated 15-22 kDa type I member of the REG gene family of proteins. It is induced in proliferating cell types such as colonic epithelium, islet beta -cells, and multiple tumor types, and is constitutively expressed by pancreatic acinar cells. Thus, Reg1A is found both in blood (ng/mL quantities) and pancreatic secretions. It would appear that Reg1A plays a role in blocking apoptosis, and is upregulated by both IL-22 and IL-6. Human Reg1A is synthesized as a 166 amino acid (aa) precursor. It contains a 22 aa signal sequence plus a 144 aa mature region that is characterized by the presence of three intrachain disulfide bonds, one C-type lectin domain (aa 34-164), and a utilized N-terminal O-linked glycosylation site (Thr27). Variablility in the glycosylation pattern, plus proteolytic cleavage after Arg11 generates Reg1A isoforms in the 15-22 kDa range. There is one potential isoform variant that shows an alternative start site at Met9. Mature (aa 23-166) human Reg1A shares 76% and 69% aa sequence identity with mouse and rat Reg1A, respectively.

Reg1B, also known as secretory pancreatic stone protein 2 and lithostathine 1 beta, is a type I subclass member of the Reg family, which comprises secreted proteins with a C-type lectin domain. Reg1B is highly related to Reg1A, a protein that is implicated in islet cell regeneration and diabetogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.