Red Zymosan Phagocytosis Assay Kit (Fluorometric)

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Product Details

Summary
Reactivity Hu, Mu, Rt, MaSpecies Glossary
Suitable Sample Type
Phagocytic cell culture: adherent or suspension cells capable of phagocytosis

Order Details

Red Zymosan Phagocytosis Assay Kit (Fluorometric) Summary

Description
Non-radioactive, high-throughput assay to quantify phagocytosis or screening phagocytosis effectors.
  • Detection method: Fluorescence (Ex/Em = 540/570 nm)
  • Applications: Rapid detection, quantification and validation of phagocytosis in convenient 96-well format. Tracking ligand internalization and screening for effectors of phagocytosis.
Kit Type
Assay Kit (Fluorometric)

Packaging, Storage & Formulations

Storage
Store at 4C.

Kit Components

Components
  1. Phagocytosis Assay Buffer
  2. 10X Quenching Solution
  3. Red Zymosan

Background

Phagocytosis in mammals serves as an important first line defense mechanism against invading pathogens. It is also essential for continuous clearance of dying cells, tissue remodeling, and acquisition of nutrients for some cells. Phagocytosis is a specific form of endocytosis initiated by recognition and binding of foreign particles by cell surface receptors, followed by their engulfment, and formation of phagosomes. Maturing phagosomes transform to phagolysosomes which destroy the pathogen through enzymes and toxic peroxides. Zymosan prepared from yeast cell wall (Saccharomyces cerevisiae), and consisting of protein-carbohydrate complexes is frequently used as a pathogen in phagocytosis assays. The Phagocytosis Assay Kit utilizes pre-labeled Zymosan particles as a tool for rapid and accurate detection and quantification of in vitro phagocytosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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