Red Zymosan Phagocytosis Assay Kit

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Product Details

Summary
Reactivity Hu, Mu, Rt, MaSpecies Glossary
Suitable Sample Type
Phagocytic cell culture: adherent or suspension cells capable of phagocytosis

Order Details

Red Zymosan Phagocytosis Assay Kit Summary

Description
EZCellTM Phagocytosis Assay Kit (Red Zymosan): Non-radioactive, high-throughput assay quantify Phagocytosis or screening Phagocytosis effectors. 100 Assays

  • Detection method- Fluorescence (400 nm excitation filter and 505 nm emission filter)
  • Applications: Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.
Kit Type
Assay Kit

Applications/Dilutions

Application Notes
Phagocytosis in mammals serves as an important first line defense mechanism against invading pathogens. It is also essential for continuous clearance of dying cells, tissue remodeling, and acquisition of nutrients for some cells. Phagocytosis is a specific form of endocytosis initiated by recognition and binding of foreign particles by cell surface receptors, followed by their engulfment, and formation of phagosomes. Maturing phagosomes transform to phagolysosomes which destroy the pathogen through enzymes and toxic peroxides. Zymosan prepared from yeast cell wall (Saccharomyces cerevisiae), and consisting of protein-carbohydrate complexes is frequently used as a pathogen in phagocytosis assays. The EZCellTM Phagocytosis Assay Kit (Green Zymosan) utilizes pre-labeled Zymosan particles as a tool for rapid and accurate detection and quantification of in vitro phagocytosis by fluorescent microscope, spectrophotometer or flow cytometry. Our kit provides a robust screening system for activators and/or inhibitors of phagocytosis and Toll-like receptors ligands (TLR)

Packaging, Storage & Formulations

Storage
Store at 4C.

Kit Components

Components
  1. 10X Quenching Solution
  2. Phagocytosis Assay Buffer
  3. Red Zymosan

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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Array NBP2-54860

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