Recombinant Viral Zika Virus (H/PF/2013) NS1 Protein, CF

• Reactivity: V
• Format: Carrier-Free

Recombinant Viral Zika Virus (H/PF/2013) NS1 Protein, CF Summary

• Details of Functionality: Bioassay data are not available.
• Source: Human embryonic kidney cell, HEK293-derived viral Zika Virus (H/PF/2013) NS1 protein
  Val796 & Ser799-Ser1148, with a C-terminal 6-His tag
• Accession #: AHZ13508
• N-terminal Sequence: Val796 & Ser799
• Protein/Peptide Type: Innovator Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity not tested
• Theoretical MW: 41 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 42-61 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Reconstitution Instructions: Reconstitute at 250 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Viral Zika Virus (H/PF/2013) NS1 Protein, CF

• Zika Virus (H/PF/2013) NS1

Background

Zika Virus non-structural protein 1 (ZIKV NS1) is an approximately 48 kDa viral glycoprotein (1). Zika Virus is a mosquito-borne flavivirus and has been implicated an association with neonatal microcephaly and neurological disorders such as Guillain-Barré syndrome (2). ZIKV strain H/PF/2013 was isolated from human host.  ZIKV NS1 is a multifunctional virulence factor. The glycosylated NS1 exists as a membrane-associated dimer after translocation into the endoplasmic reticulum lumen, where it is essential for viral genome replication (1, 2). Infected cells secrete NS1 as a lipoprotein, which is involved in immune evasion and pathogenesis via interaction with components of the innate and adaptive immune systems (1, 2). Mature ZIKV NS1 contains 352 amino acids (aa) and has a hexameric conformation consisting of three dimers to form a symmetric barrel shape (1). It has high structural similarity to other flavivirus NS1 proteins, such as DENV and WNV (2, 3). Mature NS1 of ZIKV strain H/PF/2013 shares 98% aa sequence identity with NS1 of ZIKV strain MR 766 that was isolated from monkey host.

1. Song, H. et al. (2016) Nat. Struct. and Mol. Biol. 23:456.
2. Brown, WC. et al. (2016) Nat. Struct. and Mol. Biol. 23: 865.
3. Baronti, C. et al. (2014) Genome Announc. 2: e00500

Bioinformatics

• Uniprot:
  • AHZ13508()