Recombinant Viral wnvNS1 His-tag Protein, CF

2 μg/lane of Recombinant Viral wnvNS1 His-tag Protein (Catalog # 10945-SE) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 52-62 kDa.

• Reactivity: V
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Viral wnvNS1 His-tag Protein, CF Summary

• Details of Functionality: Bioassay data are not available.
• Source: Human embryonic kidney cell, HEK293-derived viral wnvNS1 Protein protein
  Asp1-Ala352, with a C-terminal 6-His tag
• Accession #: NP_776015.1
• N-terminal Sequence: Asp1
• Protein/Peptide Type: Recombinant Enzymes
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 40 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 52-62 kDa, under reducing conditions.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Viral wnvNS1 His-tag Protein, CF

• wnvNS1 Protein

Background

The West Nile Virus [WNV] is a neurotropic human pathogen. This virus belongs to the flavivirus genus which is in the Flaviviridae family along with the Dengue virus (DENV), Yellow fever virus (YFV), Japanese encephalitis virus (JEV), and Zika virus. The West Nile Virus is a mosquito-borne virus and affects many other animals besides humans, such as birds, reptiles and other mammals (1). The West Nile Virus is an enveloped virion that contains a positive single stranded RNA. The viral RNA is translated to produce 3 structural and 7 nonstructural proteins. The structural proteins are capsid (C), envelope (E), and precursor membrane proteins (prM) and the nonstructural proteins include NS1, NS2A, NS2B, NS3, NS4A, NS4B, and NS5. The structural proteins are encoded at the 5' end of the RNA strand and serve the function of virus entry and encapsulation of the genome (2). WNV NS1 protein is a glycoprotein ~50 kDa. It shares 55.7% and 54.7% homology with NS1 from DENV2 and Zika virus. NS1 exists as a monomer, dimer, and hexamer, depending on its localization (3-5). Glycosylation is critical for its functions (6). Although no known role in viron assembly, NS1 is important for virulence and viral replication (7). Intracellular NS1 is reported to interact with various host proteins, such as 60S ribosome subunit, hnRNP C1/C2 and hnRNP K to assist virus replication (8-10). TLR3 receptor is reported to mediate WNV entry into the brain (11). Secreted and cell surface associated NS1 is highly immunogenic, making it an important biomarker and vaccine target (12-14).

1. Lindenbach, B.D. et al. (2003) Adv. Virus Res. 59:23
2. Rastogi, M. et al. (2016) Virol. J. 13:131.
3. Winkler, G. et al. (1988) Virology. 162:187.
4. Macdonald, J. et al. (2005) J. Virol. 79:13924.
5. Akey, D.L. et al. (2014) Science 343:881.
6. Somnuke, P. et al. (2011) Virology 413:253.
7. Akey, D.L. et al. (2015) Bioessays 37:489.
8. Cervantes-Salazar, M. et al. (2015) Virology 484:113.
9. Noisakran, S. et al. (2008) Biochem Biophys Res Commun. 372:67.
10. Kanlaya, R. et al. (2010) Mol Biosyst. 6:795.
11. Wang, T. et al. (2004) Nat Med. 10:1366.
12. Watterson, D. et al. (2016) Antiviral Res. 130:7.
13. Sun, E. et al. (2013) Vet Microbiol. 166:145.
14. Wu, S.F. et al. (2003) Vaccine. 21:3919.

Bioinformatics

• Uniprot:
  • NP_776015.1()