Recombinant Viral MCV-type II Chemokine-like Protein Protein

• Reactivity: V
• Applications: Bioactivity

Recombinant Viral MCV-type II Chemokine-like Protein Protein Summary

• Details of Functionality: Measured by its ability to block I-309-induced chemotaxis of BaF3 mouse pro‑B cells transfected with human CCR8. The ED₅₀ for this effect is 15‑75 ng/mL in the presence of 20 ng/mL
  rhI-309.
• Source: E. coli-derived viral MCV-type II Chemokine-like Protein protein
  Leu25-Leu104
• Accession #: AAB72041
• N-terminal Sequence: Leu25
• Protein/Peptide Type: Recombinant Proteins
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 9.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Viral MCV-type II Chemokine-like Protein Protein

• MCVtype II
• MCV-type II

Background

Molluscum contagiosum virus (MCV) is a human cutaneous poxvirus which causes benign proliferative lesions of the skin in normal and immunocompromised individuals. Based on endonuclease cleavage patterns of viral DNA, MCV isolates can be classified into two different types, MCV type I (MCVI) and MCV type II (MCVII). Like other DNA viruses such as poxviruses and herpesviruses, MCV has been shown to encode homologs of cellular immunomodulatory proteins. These viral proteins enable the virus to evade the host immune system and enhance the ability of the virus to propogate and survive.

MC148R2 is a CC chemokine-like protein encoded by a MCV type II gene. MC148R2 cDNA encodes a putative 104 amino acid (aa) protein containing a 24 aa signal peptide that is cleaved to produce an 80 aa mature protein. The mature protein shares 89% homology to the MCV type I chemokine-like protein, MC148R1. Compared to MIP-beta , MC148R2 retains the 4 conserved cysteine residues but has a 5 aa residue deletion at the amino-terminus region which is important for receptor activation. The MCV type I and type 2 proteins do not exhibit chemotactic properties, however these proteins have been shown in vitro to inhibit the monocyte chemotaxis induced by human MIP-1 alpha as well as the binding of radiolabeled I-309 to hCCR8 transfected cells.

1. Krathwohl, M. et al. (1997) Proc. Natl. Acad. Sci. USA 94:9875.
2. Senkevich, T. et al. (1996) Science 273:813.
3. Dairaghi, D.J. et al. (1999) J. Biol. Chem. 274:21569.

Publications for MCV-type II Chemokine-like Protein (697-M3)(1)

Publication using 697-M3

• Najarro P, Lee HJ, Fox J, Pease J, Smith GL Yaba-like disease virus protein 7L is a cell-surface receptor for chemokine CCL1. J. Gen. Virol., 2003-12-01;84(0):3325-36. 2003-12-01 [PMID: 14645913] (Bioassay, Virus)

Bioinformatics

• Uniprot:
  • AAB72041()