Recombinant Viral JEV-NS1 Protein, CF

• Format: Carrier-Free

Recombinant Viral JEV-NS1 Protein, CF Summary

• Details of Functionality: Bioassay data are not available.
• Source: Human embryonic kidney cell, HEK293-derived viral Japanese Encephalitis virus NS1 protein
  Thr796-Ala1146, with C-terminal 6-His tag
• Accession #: P32886
• N-terminal Sequence: Thr796
• Protein/Peptide Type: Innovator Recombinant Proteins
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity not tested
• Theoretical MW: 41 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 45-52 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Reconstitution Instructions: Reconstitute at 250 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Viral JEV-NS1 Protein, CF

• Japanese Encephalitis virus NS1
• Japanese Encephalitis
• JE virus
• JEV
• JEVgp1
• JEV-NS1

Background

Japanese encephalitis virus (JEV) non-structural protein 1 (NS1) is produced by the Japanese encephalitis virus (JEV), a mosquito-borne flavivirus that is the leading cause of virus-induced encephalitis globally (1). Flavivirus NS1 is a multifunctional virulence factor. The glycosylated NS1 exists as a membrane-associated dimer after translocation into the endoplasmic reticulum lumen, where it is essential for viral genome replication (2). The secreted hexamer NS1 is involved in immune evasion and pathogenesis; it is identified as a potential diagnostic marker for early detection of the virus infections (2). Mature viral JEV NS1 contains 352 amino acids and shares high structural similarity to other flavivirus NS1 proteins, such as DENV and ZIKV.

1. Nain, M. et al. (2016) Rev. Med. Virol. 26:129.
2. Rastogi, M. et al. (2016) Virol. J. 13:131.

Bioinformatics

• Uniprot:
  • P32886()