Recombinant Viral HPV E6 Protein, CF

• Reactivity: V
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Viral HPV E6 Protein, CF Summary

• Details of Functionality:

  Typical concentration to support E6AP-mediated p53 ubiquitination in vitro is 0.1-2 μM depending on experimental conditions.

• Source: E. coli-derived viral E6 protein
  Met1-Leu158 with a N-terminal Ser-His
  Contains Cys to Ser substitutions at the following positions: 23, 58, 87, 104, 118, 147
• Accession #: P03126.1
• Protein/Peptide Type: Recombinant Proteins
• Gene: E6
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 19 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
• Buffer: Supplied as a solution in HEPES, NaCl, DTT.
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Viral HPV E6 Protein, CF

• E6
• HpV16gp1

Background

E6 (Early protein 6) is a viral protein produced in cells infected with the Human Papillomavirus. E6 forms a complex with the host cell ubiquitin ligase E6AP (E6-Associated-Protein) generating a ligase activity that polyubiquitinates tumor suppressors p53 and p73 and targets them to the 26S proteasome for degradation. As a result DNA damage and chromosomal instabilities increase, often leading to cell proliferation and cancer. The E6/E6AP complex also targets other substrates for ubiquitination, such as TERT, BAK1, FADD, and  pro-CASP8—none of which appear to be substrates for E6AP in the absence of E6. 

1. Beaudenon S. & Huibregtse J.M. (2008) BMC Biochem. 9 (Supp1): S4.
2. Zanier K., et al. (2007) Prot. Exp. & Purif. 51: 59.
3. Zanier K., et al. (2010) J. Mol. Biol. 396: 90.

Publications for E6 (AP-120)(1)

Publication using AP-120

• Y Wang, X Liu, L Zhou, D Duong, K Bhuripanyo, B Zhao, H Zhou, R Liu, Y Bi, H Kiyokawa, J Yin Identifying the ubiquitination targets of E6AP by orthogonal ubiquitin transfer Nat Commun, 2017-12-20;8(1):2232. 2017-12-20 [PMID: 29263404] (Bioassay, Yeast)

Bioinformatics

• Gene Symbol: E6
• Uniprot:
  • P03126.1()