Recombinant Viral HCV E2 Protein, CF

Images

 
When Recombinant Human CD81 LEL Fc Chimera (Catalog # 9144-CD) is coated onto a microplate at 2 μg/ml, Recombinant Viral HCV E2 (Catalog # 9146-HC) bindswith an ED50 of 0.2-1.2 µg/mL.

Product Details

Summary
Reactivity VSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Viral HCV E2 Protein, CF Summary

Additional Information
Hepatitis C Virus 1b Envelope Glycoprotein E2
Details of Functionality
Measured by its binding ability in a functional ELISA. When Recombinant Human CD81 LEL Fc Chimera (Catalog # 9144-CD) is coated onto a microplate at 2 μg/mL, Recombinant Viral HCV E2 binds with an ED50 of 0.2-1.2 µg/mL.
Source
Human embryonic kidney cell, HEK293-derived viral HCV E2 protein
Glu384-Glu661, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Glu384
Protein/Peptide Type
Recombinant Proteins
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
31 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
63-74 kDa, reducing conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Viral HCV E2 Protein, CF

  • HCV E2
  • HCV-E2
  • hcvGP-E2
  • Hep C E2

Background

Glycoprotein E2 is an approximately 60 kDa viral envelope protein encoded by the hepatitis C virus (HCV) genome (1-3). HCV infection is closely associated with the development of hepatocellular carcinoma and cirrhosis of the liver. E2 is a transmembrane molecule expressed as a noncovalent dimer with the E1 glycoprotein (4). It binds to CD81 and CD36/SR-BI on hepatocytes, and glycosylation of E2 is important for this interaction (4-8). The virus also requires target cell expression of the tight junction proteins Claudin-1 and Occludin for infectivity (7, 9, 10). CD81 and Occludin are responsible for the human host cell specificity of HCV (10). E2 binding to CD81 on NK cells inhibits cytokine production and cytolytic activity (11, 12). In contrast, E2 binding to CD81 on T cells provides a costimulatory signal (11, 12). On plasmacytoid dendritic cells, E2 binds to CD81 as well as BDCA-2 and DCIR, resulting in inhibition of IFN-alpha production and cell maturation (13, 14).
  1. Khan, A.G. et al. (2015) Curr. Opin. Virol. 12:53.
  2. Zona, L. et al. (2014) Viruses 6:875.
  3. Grakoui, A. et al. (1993) J. Virol. 67:1385.
  4. Op De Beeck, A. et al. (2004) J. Virol. 78:2994.
  5. Pileri, P. et al. (1998) Science 282:938.
  6. Scarselli, E. et al. (2002) EMBO J. 21:5017.
  7. Harris, H.J. et al. (2010) J. Biol. Chem. 285:21092.
  8. Falkowska, E. et al. (2007) J. Virol. 81:8072.
  9. Evans, M.J. et al. (2007) Nature 446:801.
  10. Ploss, A. et al. (2009) Nature 457:882.
  11. Crotta, S. et al. (2002) J. Exp. Med. 195:35.
  12. Tseng, C.-T. and G.R. Klimpel (2002) J. Exp. Med. 195:43.
  13. Tu, Z. et al. (2013) Cell. Immunol. 284:98.
  14. Florentin, J. et al. (2012) Blood 120:4544.

Publications for HCV E2 (9146-HC) (0)

There are no publications for HCV E2 (9146-HC).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for HCV E2 (9146-HC) (0)

There are no reviews for HCV E2 (9146-HC). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for HCV E2 (9146-HC) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional HCV E2 Products

Blogs on HCV E2

There are no specific blogs for HCV E2, but you can read our latest blog posts.

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Viral HCV E2 Protein, CF and receive a gift card or discount.

Bioinformatics

Uniprot