Recombinant Viral Dengue Virus 1 NS1 Protein, CF

• Reactivity: V
• Format: Carrier-Free

Recombinant Viral Dengue Virus 1 NS1 Protein, CF Summary

• Details of Functionality: Bioassay data are not available.
• Source: Human embryonic kidney cell, HEK293-derived viral Dengue Virus 1 NS1 protein
  Asp776-Ala1127, with a C-terminal 7-His tag
• Accession #: P17763
• N-terminal Sequence: Asp776
• Protein/Peptide Type: Innovator Recombinant Proteins
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity not tested
• Theoretical MW: 41 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 44-54 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Viral Dengue Virus 1 NS1 Protein, CF

• DEN-1
• Dengue Virus 1 NS1
• Dengue virus Serotype 1
• Dengue virus Type 1
• DENV1
• DENV1-NS1

Background

Dengue virus type 1 non-structural protein 1 (DENV1 NS1)  is an approximately 50 kDa viral glycoprotein (1). Dengue viruses are mosquito-borne flaviviruses that exist in nature as four distinct serotypes (DENV1-4) (2). Dengue virus NS1 can be found associated with cell membranes, within cells and at the cell surface, or as secreted extracellular species. The secreted form of NS1 is known to be hexameric (1). Within cells, NS1 plays a significant role in virus replication and has been shown to co-localize with dsRNA and other components of viral replication complexes in viral-induced vesicle packets (1,3). Cell surface-associated and secreted NS1 are profoundly immunogenic, and both the protein itself and the antibodies it elicits have been shown to contribute to either protection or pathogenesis and innate immune evasion (1, 3). Mature DENV1 NS1 contains 352 amino acids (aa) and consists of 2 conserved N-glycosylation sites and 12 invariant cysteine residues that form six intramolecular disulfide bonds (3).Mature DENV1 NS1 shares 73%, 80%, and 69% aa sequence identity with DENV2, DENV3, and DENV4 NS1, respectively.

1. Muller, D. et al. (2012) J. Gen. Virol. 93:771.
2. VanBlargan, L.A. et al. (2013) PLoS Pathog. 9:e1003761.
3. Flamand, M. et al. (1999) J. Virol. 73:6104.

Bioinformatics

• Uniprot:
  • P17763()