Recombinant Viral CKBP Protein, CF

• Reactivity: V
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Viral CKBP Protein, CF Summary

• Details of Functionality: Measured by its ability to inhibit CCL3/MIP-1 alpha -induced chemotaxis of BaF3 mouse pro‑B cells transfected with human CCR5. The ED₅₀ for this effect is 0.02-0.1 μg/mL.
• Source: Human embryonic kidney cell, HEK293-derived viral CKBP protein
  Pro18-Val258, with an N-terminal 6-His tag
• Accession #: P19063
• N-terminal Sequence: His
• Protein/Peptide Type: Recombinant Proteins
• Purity: >90%, by SDS-PAGE with silver staining
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 27 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 36-40 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >90%, by SDS-PAGE with silver staining
• Reconstitution Instructions: Reconstitute at 500 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Viral CKBP Protein, CF

• B29R
• C23L
• CKBP

Background

Chemokine-binding Protein (CKBP), also known as, CKBP-II, vCCI, or B29R, is a 35 kDa secreted protein encoded by multiple poxviruses, including the Vaccinia virus (Lister strain) (1). While CKBP binds and sequesters CC chemokines with high affinity, it shares little amino acid sequence identity with CC chemokine receptors (1-3). CKBP prevents CC chemokine activity by masking the chemokine/chemokine receptor binding site (4). Chemokine-binding proteins encoded by poxvirus and herpesvirus strains inhibit the recruitment of immune cells and contribute to viral evasion of host immune responses (5). Local administration of pan-CC chemokine binding proteins such as CKBP have been shown to reduce inflammation resulting from allergic airway hyperactivity and arthritis (6, 7).

1. Alcami, A. et al. (1998) J. Immunol. 160:624.
2. Kuo, N.W. et al. (2014) J. Biol. Chem. [In press].
3. Zhang, L. et al. (2006) Proc. Natl. Acad. Sci. USA 103:13985.
4. Beck, C.G. et al. (2001) J. Biol. Chem. 276:43270.
5. Seet, B.T. and G. McFadden (2002) J. Leukoc. Biol. 72:24.
6. Dabbagh, K. et al. (2000) J. Immunol. 165:3418.
7. Buatois, V. et al. (2010) J. Immunol. 185:2544.

Bioinformatics

• Uniprot:
  • P19063(Viral)