Recombinant Viral CKBP Protein, CF

Images

 
There are currently no images for CKBP (8254-CK/CF).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity VSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

View Available Formulations
Catalog# & Formulation Size Price

Recombinant Viral CKBP Protein, CF Summary

Details of Functionality
Measured by its ability to inhibit CCL3/MIP-1 alpha -induced chemotaxis of BaF3 mouse pro‑B cells transfected with human CCR5. The ED50 for this effect is 0.02-0.1 μg/mL.
Source
Human embryonic kidney cell, HEK293-derived viral CKBP protein
Pro18-Val258, with an N-terminal 6-His tag
Accession #
N-terminal Sequence
His
Protein/Peptide Type
Recombinant Proteins
Purity
>90%, by SDS-PAGE with silver staining
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
36-40 kDa, reducing conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>90%, by SDS-PAGE with silver staining
Reconstitution Instructions
Reconstitute at 500 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Viral CKBP Protein, CF

  • B29R
  • C23L
  • CKBP

Background

Chemokine-binding Protein (CKBP), also known as, CKBP-II, vCCI, or B29R, is a 35 kDa secreted protein encoded by multiple poxviruses, including the Vaccinia virus (Lister strain) (1). While CKBP binds and sequesters CC chemokines with high affinity, it shares little amino acid sequence identity with CC chemokine receptors (1-3). CKBP prevents CC chemokine activity by masking the chemokine/chemokine receptor binding site (4). Chemokine-binding proteins encoded by poxvirus and herpesvirus strains inhibit the recruitment of immune cells and contribute to viral evasion of host immune responses (5). Local administration of pan-CC chemokine binding proteins such as CKBP have been shown to reduce inflammation resulting from allergic airway hyperactivity and arthritis (6, 7).
  1. Alcami, A. et al. (1998) J. Immunol. 160:624.
  2. Kuo, N.W. et al. (2014) J. Biol. Chem. [In press].
  3. Zhang, L. et al. (2006) Proc. Natl. Acad. Sci. USA 103:13985.
  4. Beck, C.G. et al. (2001) J. Biol. Chem. 276:43270.
  5. Seet, B.T. and G. McFadden (2002) J. Leukoc. Biol. 72:24.
  6. Dabbagh, K. et al. (2000) J. Immunol. 165:3418.
  7. Buatois, V. et al. (2010) J. Immunol. 185:2544.

Publications for CKBP (8254-CK/CF) (0)

There are no publications for CKBP (8254-CK/CF).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for CKBP (8254-CK/CF) (0)

There are no reviews for CKBP (8254-CK/CF). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for CKBP (8254-CK/CF) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional CKBP Products

Array 8254-CK/CF

Blogs on CKBP

There are no specific blogs for CKBP, but you can read our latest blog posts.

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Viral CKBP Protein, CF and receive a gift card or discount.

Bioinformatics

Uniprot