Recombinant Viral CCI Fc Chimera Protein, CF

Recombinant Viral CCI Fc Chimera (Catalog # 696-CC) inhibits Recombinant Mouse CCL2/JE/MCP-1 (Catalog # 479-JE) induced chemotaxis of BaF3 mouse pro-B cells transfected with human CCR2A. The ED50 for this effect is0.1-0.5 µg/mL in the presence of 20 ng/mL Recombinant Mouse CCL2/JE/MCP-1.

• Reactivity: V
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Viral CCI Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its ability to inhibit JE-induced chemotaxis of BaF3 mouse pro‑B cells transfected with human CCR2A. The ED₅₀ for this effect is 0.1-0.5 µg/mL in the presence of 20 ng/mL of recombinant mouse JE.
• Source: Spodoptera frugiperda, Sf 21 (baculovirus)-derived viral CCI protein
  

  Viral CCI (Met17-Val258)& (Pro18-Val258) Accession # P19063	IEGRMD	Human IgG1 (Pro100-Lys330)	6-His tag
  N-terminus			C-terminus

• Accession #: P19063
• N-terminal Sequence: Met17 & Pro18
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 57 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Viral CCI Fc Chimera Protein, CF

• CCI

Background

The family of T1/35 kDa proteins are encoded by the '35K' virulence gene of many poxviruses and are secreted from virus-infected cells. These proteins bind
CC-chemokines with high affinity and are termed viral chemokine inhibitor (vCCI). Viral CCI from various poxviruses share multiple stretches of sequence identity and eight conserved cysteine residues (1). The vaccinia virus (strain Lister) vCCI cDNA encodes a 258 amino acid (aa) protein with a putative 17 aa signal peptide (2). Vaccinia virus (strain Lister) vCCI shows greater than 90% aa sequence identity with vCCI from other orthopoxviruses and approximately 40% aa sequence identity with the leporipoxvirus T-1 proteins. vCCI binds with high affinity to many human, mouse, and rat CC-chemokines (1, 3-5). It binds to the same surfaces of these chemokines that are involved in chemokine receptor interactions (4-7). Some CC-chemokines utilize common binding surfaces on vCCI, while others interact with distinct sites on vCCI (8). vCCI inhibits CC-chemokine induced monocyte chemotaxis in vitro but does not block cellular effects induced by CXCL5/ENA-78, CXCL8/IL-8, or CXCL10/IP-10 (3-5). In vivo, vCCI restricts the infiltration of  immune cells into sites of inflammation (3, 9, 10) and also the CC-chemokine induced migration of arterial vascular smooth muscle cells (11).

1. Graham, K.A. et al. (1997) Virology 229:12.
2. Patel, A.H. et al. (1990) J. Gen. Virol. 71:2013.
3. Buatois, V. et al. (2010) J. Immunol. 185:2544.
4. Smith, C.A. et al. (1997) Virology 236:316.
5. Lalani, A.S. et al. (1998) Virology 250:173.
6. Beck, C.G. et al. (2001) J. Biol. Chem. 276:43270.
7. Seet, B.T. et al. (2001) Proc. Natl. Acad. Sci. 98:9008.
8. Burns, J.M. et al. (2002) J. Biol. Chem. 277:2785.
9. Dabbagh, K. et al. (2000) J. Immunol. 165:3418.
10. Reading, P.C. et al. (2003) J. Immunol. 170:1435.
11. Spinetti, G. et al. (2004) Arterioscler. Throm. Vasc. Biol. 24:1397.

Publications for CCI (696-CC)(1)

Publication using 696-CC

• Buatois V, Fagete S, Magistrelli G, Chatel L, Fischer N, Kosco-Vilbois MH, Ferlin WG Pan-CC chemokine neutralization restricts splenocyte egress and reduces inflammation in a model of arthritis. J. Immunol., 2010-07-19;185(4):2544-54. 2010-07-19 [PMID: 20644170] (ELISA (Capture), Human)

Bioinformatics

• Uniprot:
  • P19063(Viral)