Recombinant SARS-CoV Nucleocapsid His-tag Protein, CF

• Reactivity: V
• Format: Carrier-Free

Recombinant SARS-CoV Nucleocapsid His-tag Protein, CF Summary

• Details of Functionality: Bioassay data are not available.
• Source: Spodoptera frugiperda, Sf 21 (baculovirus)-derived sars-cov Nucleocapsid protein
  Met1-Ala422, with a C-terminal 6-His tag
• Accession #: NP_828858.1
• N-terminal Sequence: Ser2, determined by protein ID
• Protein/Peptide Type: Recombinant Proteins
• Purity: >85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity not tested
• Theoretical MW: 47 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 41-55 kDa, under reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS and EDTA with Trehalose.
• Purity: >85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 1 mg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant SARS-CoV Nucleocapsid His-tag Protein, CF

• N Protein
• Nucleocapsid protein
• Nucleocapsid
• ORF9a protein

Background

SARS-CoV was discovered in association with cases of severe acute respiratory syndrome (SARS) that infected more than 8,000 persons with over 900 fatalities worldwide in 2002-2003 (1). It belongs to a family of viruses known as coronaviruses that also include MERS and SARS-Cov2 that causes the global pandemic coronavirus disease 2019 (Covid-19). Coronavirus is commonly comprised of four structural proteins: Spike protein(S), Envelope protein (E), Membrane protein (M), and Nucleocapsid protein (N) (1). While the S, E and M proteins build up the viral envelop, the N protein is involved in transcription, replication, and packaging of the viral RNA genome into a helical ribonucleocapsid (RNP) (2, 3). The SARS-CoV N protein is a ~45 kDa protein composed of two independent structural domains connected by a linker region. Both the N-terminal and the linker regions contain RNA binding domains, while the C-terminal region is responsible for the oligomerization of the N protein (2, 3). The SARS-CoV N protein shares 90% amino acid sequence identity with SARS-CoV-2 N protein. The N protein is an abundant protein during coronavirus infection and displays high immunogenic activity (4, 5), so it has been used to develop serological diagnostic kit for SARS IgM and IgG antibody tests.

1. Rota, P.A. et al. (2003) Science 300:1394.
2. Chang, C.K. et al. (2006) J.Biomed. Sci. 13:59.
3. Hurst, K. R. et al. (2009) J. Virol. 83:7221.
4. Che, X. Y. et al. (2004) J. Clin. Microbiol. 42:2629.
5. Guan, M. et al. (2004) Clin. Diagn. Lab. Immunol. 11:287.

Bioinformatics

• Uniprot:
  • NP_828858.1()