Recombinant SARS-CoV-2 Papain-like Protease, CF

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0.5 μg and 1 μg of Recombinant SARS-CoV-2 Papain-like Protease (Catalog # E-611) was resolved with SDS-PAGE under reducing conditions and visualized by colloidal Coomassie Blue staining, showing a band at 57 kDa.
0.5 μg and 1 μg of Recombinant SARS-CoV-2 Papain-like Protease (Catalog # E-611) was resolved with SDS-PAGE under reducing conditions and visualized by colloidal Coomassie Blue staining, showing a band at 57 kDa.
0.5 μg and 1 μg of Recombinant SARS-CoV-2 Papain-like Protease (Catalog # E-611) was resolved with SDS-PAGE under reducing conditions and visualized by colloidal Coomassie Blue staining, showing a band at 57 kDa.
0.5 μg and 1 μg of Recombinant SARS-CoV-2 Papain-like Protease (Catalog # E-611) was resolved with SDS-PAGE under reducing conditions and visualized by colloidal Coomassie Blue staining, showing a band at 57 kDa.
0.5 μg and 1 μg of Recombinant SARS-CoV-2 Papain-like Protease (Catalog # E-611) was resolved with SDS-PAGE under reducing conditions and visualized by colloidal Coomassie Blue staining, showing a band at 57 kDa.
0.5 μg and 1 μg of Recombinant SARS-CoV-2 Papain-like Protease (Catalog # E-611) was resolved with SDS-PAGE under reducing conditions and visualized by colloidal Coomassie Blue staining, showing a band at 57 kDa.
0.5 μg and 1 μg of Recombinant SARS-CoV-2 Papain-likeProtease (Catalog # E-611) was resolved with SDS-PAGE under reducing conditionsand visualized by colloidal Coomassie Blue staining, showing a band at 57 kDa.
0.5 μg and 1 μg of Recombinant SARS-CoV-2 Papain-likeProtease (Catalog # E-611) was resolved with SDS-PAGE under reducing conditionsand visualized by colloidal Coomassie Blue staining, showing a band at 57 kDa.
0.5 μg and 1 μg of Recombinant SARS-CoV-2 Papain-like Protease (Catalog # E-611) was resolved with SDS-PAGE under reducing conditions and visualized by colloidal Coomassie Blue staining, showing a band at 57 kDa.
0.5 μg and 1 μg of Recombinant SARS-CoV-2 Papain-like Protease (Catalog # E-611) was resolved with SDS-PAGE under reducing conditions and visualized by colloidal Coomassie Blue staining, showing a band at 57 kDa.
0.5
0.5 μg and 1 μg of Recombinant SARS-CoV-2 Papain-like Protease (Catalog # E-611) was resolved with SDS-PAGE under reducing conditions and visualized by colloidal Coomassie Blue staining, showing a band at 57 kDa.

Product Details

Summary
Reactivity VSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant SARS-CoV-2 Papain-like Protease, CF Summary

Additional Information
View All of the Proteins for Coronavirus Research
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Details of Functionality
Recombinant SARS-CoV-2 virus Papain-like protease (PLPro) is a Ubiquitin- and ISG15-deconjugating enzyme.  Reaction conditions will need to be optimized for each specific application.  We recommend an initial PLPro concentration of 20-100 nM when using Ubiquitin-Rhodamine 110 (U-555) substrate.  When using polyubiquitin chain substrate(s), this enzyme demonstrates a preference for K48 linkages.
Source
E. coli-derived sars-cov-2 Papain-like Protease protein
Glu746 - Lys1060
Accession #
Protein/Peptide Type
Recombinant Enzymes
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Applications/Dilutions

Theoretical MW
62 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 60 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Buffer
X mg/ml (X μM) in 50 mM HEPES pH 8.0, 300 mM NaCl, 10% (v/v) Glycerol, 1 mM TCEP
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant SARS-CoV-2 Papain-like Protease, CF

  • Papain-like Protease
  • PLpro
  • PL-PRO
  • pp1a
  • Replicase polyprotein 1a

Background

The Papain-like protease (PLPro) from the human SARS-CoV-2 coronavirus (Severe Acute Respiratory Syndrome coronavirus 2 or Wuhan 2019 coronavirus) is a cysteine protease located within the non-structural protein 3 (NS3) section of the viral polypeptide.  In other coronaviruses, PLPro activity is required to process the viral polyprotein into functional, mature subunits; specifically, PLPro cleaves a site at the amino-terminal end of the viral replicase region.  In addition to its role in viral protein maturation, PLPro possesses a deubiquitinating and deISG15ylating activity.  In vivo, this protease antagonizes innate immunity by acting on IFN beta and NF- kappa B signaling pathways.  When used in vitro with polyubiquitin substrates, the enzyme demonstrates a strong preference for K48 linkages.  This protein contains an N-terminal GST tag.
  1. Clasman J.R., et al. (2020) Antiviral Res.  174: 104661
  2. Frieman M., et al. (2009) J. Virol.  83: 6689
  3. Lindner H.A., et al. (2007) Arch. Biochem. Biophys.  466: 8
  4. Ratia K., et al. (2014) PLoS Pathog.  doi: 10.1371/journal.ppat.1004113

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