Recombinant SARS-CoV-2 NSP10 His-tag Protein, CF

2 μg/lane of Recombinant SARS-CoV-2 NSP10 His-tag (Catalog # 10630-CV) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing a band at 16 kDa under reducing conditions.

• Reactivity: V
• Format: Carrier-Free

Recombinant SARS-CoV-2 NSP10 His-tag Protein, CF Summary

• Source: E. coli-derived sars-cov-2 NSP10 protein
  Ala1-Gln139
  with a C-terminal 6-His tag
• Accession #: YP_009725306.1
• N-terminal Sequence: Ala1
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Theoretical MW: 16 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 16 kDa, under reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.
• Buffer: Supplied as a 0.2 μm filtered solution in HEPES and NaCl.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant SARS-CoV-2 NSP10 His-tag Protein, CF

• NSP10

Background

Non-structural protein 10 (NSP10), is one of several functional proteins released by ORF1a-encoded protease cleavage of the pp1a and pp1ab replicase polyproteins expressed from the coronavirus (CoV) genome (1). The NSPs are involved in the replication and transcription of the viral RNA and not incorporated within the virion particles. Coronaviruses include various highly pathogenic strains such as SARS-CoV, MERS-CoV and SARS-CoV2 that have had significant impact on humans as well as strains that have negatively impacted livestock. NSP10 is a small 139 amino acid protein that forms independent dodecameric structures composed of four identical trimers where each monomer is capable of binding two zinc ions (2, 3) that are coordinated uniquely and has exposed basic residues on the protein's surface consequently allowing RNA-binding (3). The NSP10 sequence is conserved across coronaviruses (4) and is thought to serve as an important multifunctional cofactor in viral replication. NSP10 was shown to interact with other NSP proteins including NSP14 and NSP16 (5). The N-terminus of NSP10 has been shown to interact with NSP14 and proposed to stabilize and regulate the 3'-5' exoribonuclease domain activity while not directly impacting the methyltransferase activity of NSP14 (6,7). A similar region of NSP10 interacts with NSP16; this interaction is required for stabilization of NSP16 and stimulation of its 2'-O-methyltransferase activity (4,7). Consequently, NSP10 is critically involved in the formation of functional viral mRNA capping machinery in coronaviruses and a potential target to reduce viral replication and pathogenesis (8,9). 

1. Snijder, E.J. et al. (2016) Adv. Virus Res. 96:59. 
2. Matthes, N. et al. (2006) FEBS Lett. 580:4143.
3. Su, D. et al. (2006) J. Virol. 80:7902.
4. Rosas-Lemus, M. et al. (2020) bioRxiv. In press.
5. Pan, J. et al. (2008) PLoS One 3:e3299.
6. Bouvet, M. et al. (2012) Proc. Natl. Acad. Sci. U.S.A. 109:9372.
7. Bouvet, M. et al. (2014) J. Biol. Chem. 289:25783.
8. Ma, Y. et al. (2015) Proc. Natl. Acad. Sci. 112:9436.
9. Wang, Y. et al. (2015) J. Virol. 89:8416.

Publications for NSP10 (10630-CV)(1)

Publication using 10630-CV

• Haystead, T;Lee, E;Cho, K;Gullickson, G;Hughes, P;Krafsur, G;Freeze, R;Scarneo, S; Investigation of SARS-CoV-2 individual proteins reveals the in vitro and in vivo immunogenicity of membrane protein Scientific reports 2023-12-18 [PMID: 38129491] (Bioassay, Human)

Bioinformatics

• Uniprot:
  • YP_009725306.1()