Recombinant SARS-CoV-2 NSP1 His-tag Protein, CF

2 μg/lane of Recombinant SARS-CoV-2 NSP1 His-tag (Catalog # 10666-CV) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 20-23 kDa under reducing conditions.

• Reactivity: V
• Format: Carrier-Free

Recombinant SARS-CoV-2 NSP1 His-tag Protein, CF Summary

• Source: E. coli-derived sars-cov-2 NSP1 protein
  Met1-Gly180
  with a C-terminal 6-His tag
• Accession #: YP_009725297.1
• N-terminal Sequence: Met1
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Theoretical MW: 21 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 20-23 kDa, under reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.
• Buffer: Supplied as a 0.2 μm filtered solution in HEPES and NaCl.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant SARS-CoV-2 NSP1 His-tag Protein, CF

• NSP1

Background

Non-structural protein 1 (NSP1) is one of several functional proteins released by ORF1a-encoded protease cleavage of the pp1a and pp1ab replicase polyproteins expressed from the coronavirus (CoV) genome (1). The NSPs are involved in the replication and transcription of the viral RNA and not incorporated within the virion particles. Coronaviruses include various highly pathogenic strains such as SARS-CoV, MERS-CoV and SARS-CoV2 that have had significant impact on humans as well as strains that have negatively impacted livestock. NSP1, also known as the host shutoff factor, is a small 180 amino acid highly conserved protein among the first to be expressed following cell entry.  It is composed of an N-terminal domain, a linker region, and a C-terminal domain with a conserved KH motif that is required and sufficient for specific contacts with ribosomes (2, 3). The C-terminal domain binds tightly to and sterically occludes the entrance region of the mRNA channel in free 40S subunits, 43S pre-initiation complex, and empty, non-translating 80S ribosomes (2, 3) to inhibit translation. NSP1 blocks the innate immune response through suppression of host gene expression. By preventing translation of interferon and downstream signaling responses, it plays a key role in immune evasion (2, 4, 5). NSP1 also induces endonucleolytic cleavage of host mRNAs (6,7). Concomitant translation of more efficiently recognized untranslated regions (UTR) of viral mRNA along with resistance to endonucleolytic cleavage (7) is thought to lead to a switch to production of viral mRNA over host cell mRNA during an infection (3,8). Given the critical role NSP1 plays in virulence, it is an attractive target for small-molecule inhibition and vaccination development (9, 10).  

1. Snijder, E.J. et al. (2016) Adv. Virus Res 96:59.
2. Thoms, M. et al. (2020) Science 369:1249.
3. Schubert, K. et al. (2020) Nat. Struct. Mol. Biol. 27:959.
4. Narayanan, K. et al. (2008) J. Virol. 82:4471.
5. Jauregui, A.R. et al. (2013) PLoS One. 8:e62416.
6. Kamitani, W. et al. (2006) Proc. Natl. Acad. Sci. USA 103:12885.
7. Huang, C. et al. (2011) PLoS Pathog. 7:e1002433.
8. Hartenian, E. et al. (2020) J. Biol. Chem. 295:12910. 
9. Zust, R. et al. (2007) PLoS Pathog. 3:e109.
10. De Lima Menezes, G. and R.A. da Silva (2020) J. Biomol. Struct. Dyn. (In press). 

Publications for nsp1 (10666-CV)(1)

Publication using 10666-CV

• Haystead, T;Lee, E;Cho, K;Gullickson, G;Hughes, P;Krafsur, G;Freeze, R;Scarneo, S; Investigation of SARS-CoV-2 individual proteins reveals the in vitro and in vivo immunogenicity of membrane protein Scientific reports 2023-12-18 [PMID: 38129491] (Bioassay, Human)

Bioinformatics

• Uniprot:
  • YP_009725297.1()