Recombinant R. sanguineus Evasin-3 His-tag Protein, CF

Recombinant R. sanguineus Evasin‑3 (Catalog # 10034-EV) inhibits IL‑8/CXCL8-induced chemotaxis of BaF3 mouse pro‑B cells transfected with human CCXR2. The ED50 for this effect is 0.15-0.9 μg/mL.

• Reactivity: I
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant R. sanguineus Evasin-3 His-tag Protein, CF Summary

• Details of Functionality: Measured by its ability to inhibit CXCL8-induced chemotaxis of BaF3 mouse pro-B cells transfected with human CXCR2. The ED₅₀ for this effect is 0.15-0.9 μg/mL.
• Source: Spodoptera frugiperda, Sf 21 (baculovirus)-derived r. sanguineus Evasin-3 protein
  Leu21-Arg86. with a C-terminal 6-His tag
• Accession #: P0C8E8
• N-terminal Sequence: Leu21
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 7.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 8-17 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 250 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant R. sanguineus Evasin-3 His-tag Protein, CF

• Evasin-3

Background

Evasin-3 is a highly selective chemokine-binding protein isolated from tick saliva. The cDNA of tick Evasin-3 encodes an 86 amino acid (aa) precursor, which includes a 20 aa signal peptide and a 66 aa mature protein (1). Ticks are blood sucking parasites that secrete a wide variety of immunomodulatory proteins to evade the host immune response. The saliva isolated from Ticks has shown to contain chemokine neutralization activity. These proteins have been identified as chemokine binding proteins (CHPBs) that were termed as Evasins (1,2.3). Evasin-3 belongs to a new class of chemokine binding proteins in that it shows high affinity binding to a very limited set of chemokines, including CXCL8 and CXCL1. Since it is very small molecule Evasin-3 may be therapeutically useful as novel anti-inflammatory agent.

1. Frauenschuh, A. et al. (2007) J. Biol. Chem. 282:27250.
2. Deruaz, M. et al. (2008) J. Exp. Med. 205:2019.
3. Deruaz, M. et al. (2013) FEBS J. 280:4876.

Bioinformatics

• Uniprot:
  • P0C8E8()