Recombinant Mouse VSTM5 Fc Chimera Protein, CF

Measured by its ability to inhibit anti-CD3 antibody induced IL-2 or IFN-gamma secretion by human T cells. The ED50 for this effect is 1‑6 μg/mL.

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse VSTM5 Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its ability to inhibit anti-CD3 antibody induced IL-2 or IFN-gamma secretion by human T cells. The ED₅₀ for his effect is 1-6 μg/mL.
• Source: Mouse myeloma cell line, NS0-derived mouse VSTM5 protein
  

  Mouse VSTM5 (Leu28-His146) Accession # Q9D806	IEGRMDP	Mouse IgG2a (Glu98-Lys330)
  N-terminus		C-terminus

• Accession #: Q9D806
• N-terminal Sequence: Leu28
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 41 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 51-64 kDa, under reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 200 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse VSTM5 Fc Chimera Protein, CF

• C11orf90
• V-set and transmembrane domain containing 5
• V-set and transmembrane domain-containing protein 5
• VSTM5

Background

V-set and transmembrane domain-containing protein 5 (VSTM5) is a cell-adhesion-like molecule found in the nervous system. It belongs to the VSTM family, a group of membrane proteins with an extracellular V-set domain and a single membrane-spanning region (1). Mouse VSTM5 is synthesized as a 199 amino acid (aa) precursor that contains a 27 aa signal sequence, a 119 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 32 aa cytoplasmic tail. Within the ECD, mouse VSTM5 shares 79% and 92% aa sequence identity with human and rat VSTM5, respectively. VSTM5 regulates morphology of the neurons during development by promotion of developing dendrites and forming synapse, and it guides both the positional migration of neuron cells and their dendritic morphology (1). N-linked glycosylation at multiple sites plays important roles for the expression, targeting, and function of VSTM5 (2).

1. Lee, A.R. et al. (2016) J. Neurosci. 36:10181.
2. Lee, A.R. et al. (2017) PLoS One 12:e0181257.

Bioinformatics

• Uniprot:
  • Q9D806()