Recombinant Mouse VSIG8 Fc Chimera Protein, CF

Recombinant Mouse VSIG-8 Fc Chimera (Catalog # 9204-VS)inhibits anti-CD3 antibody induced IL-2 secretion in human T lymphocytes. TheED50 for this effect is 0.5-3 μg/mL.

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse VSIG8 Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its ability to inhibit anti-CD3 antibody induced IL-2 secretion in human T lymphocytes. The ED₅₀ for this effect is 0.5-3 μg/mL.
• Source: Mouse myeloma cell line, NS0-derived mouse VSIG8 protein
  

  Mouse VSIG8 (Val22-Gly262) Accession # Q6P3A4	IEGRMDP	Mouse IgG2a (Glu98-Lys330)
  N-terminus		C-terminus

• Accession #: Q6P3A4
• N-terminal Sequence: Val22
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 54 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 56-66 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse VSIG8 Fc Chimera Protein, CF

• V-set and immunoglobulin domain containing 8
• VSIG8

Background

VSIG8 (V-set and immunoglobulin domain containing 8), also known as C1orf204, is an approximately 45 kDa type I transmembrane protein of the B7 family within the Ig superfamily. Mature mouse VSIG8 consists of a 241 amino acid (aa) extracellular domain (ECD) containing two V-type Ig-like domains, a 21 aa transmembrane domain, and a 134 aa cytoplasmic domain. Within the ECD, mouse VSIG8 shares 88% and 95% aa identity with human and rat VSIG8, respectively. Alternative splicing generates an isoform of mouse VSIG8 that lacks most of the first Ig-like domain. VSIG8 was identified from proteomic analysis of human hair shafts (1, 2). It is expressed in the hair follicle and shaft, superficial layers of the nail matrix, and superficial layers of oral epithelium (3). R&D Systems in-house testing indicates that VSIG8 inhibits the production of IL-2 by activated T cells.

1. Rice, R.H. et al. (2010) J. Proteome Res. 9:6752.
2. Lee, Y.J. et al. (2006) Mol. Cell. Proteomics 5:789.
3. Rice, R.H. et al. (2011) J. Invest. Dermatol. 131:1936.

Bioinformatics

• Uniprot:
  • Q6P3A4()