Recombinant Mouse VSIG10 Fc Chimera Protein, CF

Recombinant Mouse VSIG10 Fc Chimera (Catalog # 9604‑VS)inhibits anti-CD3 antibody induced IL-17 secretion in human PBMC.The ED50 for this effect is 0.15‑0.9 μg/mL.

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse VSIG10 Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its ability to inhibit anti-CD3 antibody induced IL-17 secretion by human peripheral blood mononuclear cells (PBMC). The ED₅₀ for this effect is 0.15-0.9 μg/mL.
• Source: Mouse myeloma cell line, NS0-derived mouse VSIG10 protein
  

  Mouse VSIG10 (Leu25-Asn426) Accession # D3YX43	IEGRMDP	Mouse IgG2a (Glu98-Lys330)
  N-terminus		C-terminus

• Accession #: D3YX43
• N-terminal Sequence: Leu25
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 71 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 97-118 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 200 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse VSIG10 Fc Chimera Protein, CF

• FLJ20674
• V-set and immunoglobulin domain containing 10
• V-set and immunoglobulin domain-containing protein 10
• VSIG10

Background

VSIG10 (V-set and immunoglobulin domain-containing protein 10) is a single-pass type I membrane protein of the Ig superfamily. Mouse VSIG10 cDNA encodes a 558 amino acid (aa) precursor that contains a 20 aa signal sequence, a 406 aa extracellular domain (ECD), a 21 aa transmembrane sequence, and a 111 aa cytoplasmic domain (1). The ECD of mouse VSIG10 contains four Ig-like C2-type domains and shares 63% and 84% aa identity with human and rat VSIG10, respectively. The specific biological function of VSIG10L has yet to be elucidated, but there is evidence to suggest the molecule is involved in cell-cell adhesion. Ig‑like C2-type domains are similar to antibody constant domains and can be found in T cell adhesion molecules, such as Cluster of Differentiation 2 (CD2) along with vascular and intercellular cell adhesion molecules (VCAM and ICAM, respectively) (2). VSIG10 is structurally related to the B7 family of immune regulatory proteins. Our studies at R&D Systems show that VSIG10 inhibits T cell activation, including IL-17 and Interferon gamma production.

1. SwissProt Accession # D3YX43.
2. Yang H. and E. L. Reinherz (2001) J. Biol. Chem. 276:18775.

Bioinformatics

• Uniprot:
  • D3YX43()