Recombinant Mouse TCAM-1 Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse TCAM-1 Protein, CF Summary

• Details of Functionality: Measured by the ability of the immobilized protein to support the adhesion of MLTC‑1 mouse Leydig tumor cells. When 5 x 10⁴ cells per well are added to TCAM-1 coated plates (5 μg/mL, 100 μL/well), approximately 65%-85% will adhere after 45 minutes at 37 °C.
• Source: Mouse myeloma cell line, NS0-derived mouse TCAM-1 protein
  Ala20-Gly483 & Glu22-Gly483
• Accession #: BAB41106
• N-terminal Sequence: Ala20 & Glu22
• Protein/Peptide Type: Recombinant Proteins
• Gene: Tcam1
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 52 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 70-80 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 200 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse TCAM-1 Protein, CF

• TCAM1
• TCAM-1

Background

Mouse TCAM-1 (Testicular cell adhesion molecule-1) is a 59 kDa (predicted) type I transmembrane protein that is a member of the intracellular adhesion molecule (ICAM) family belonging to the immunoglobulin (Ig) superfamily (1). It is synthesized from a 548 amino acid (aa) precursor that contains an 18 aa signal sequence, a 465 aa extracellular region, a 25 aa transmembrane region, and a short cytoplasmic tail of 40 aa. The extracellular region contains five Ig domains and six potential N-linked glycosylation sites (1). Mouse TCAM-1 is 89% identical to rat TCAM-1. Expression of TCAM-1 is detected in the embryo on day 17 and in adult testis, and correlates with the presence of pachytene spermatocytes and spermatids in mouse testis development (1).

1. Skatani, S. et al. (2000) Genome 43:957.

Bioinformatics

• Gene Symbol: Tcam1
• Uniprot:
  • BAB41106()