Recombinant Mouse OLT-2/TARM1 Fc Chimera Protein, CF

Recombinant Mouse OLT-2/TARM1 Fc Chimera (10000-TA) inhibits IFN-gammasecretion by human peripheral blood mononuclear cells in the presence ofanti-CD3 antibody. The ED50 for this effect is 1-10 μg/mL.

2 μg/lane of Recombinant Mouse OLT-2/TARM1 Fc Chimera was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 70-80 kDa and 140-160 kDa, respectively.

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse OLT-2/TARM1 Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its ability to inhibit anti-CD3 antibody induced IFN-gamma secretion by human peripheral blood mononuclear cells (PBMC). The ED₅₀ for this effect is 1-10 μg/mL.
• Source: Mouse myeloma cell line, NS0-derived mouse OLT-2/TARM1 protein
  

  Mouse TARM-1 (Gln17-Ile257) Accession # B6A8R8	IEGRMDP	Mouse IgG2a (Glu98-Lys330)
  N-terminus		C-terminus

• Accession #: B6A8R8
• N-terminal Sequence: No results obtained: Gln17 predicted.
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 53 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 70-80 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse OLT-2/TARM1 Fc Chimera Protein, CF

• OLT2
• OLT-2/TARM1
• OSCAR-Like Transcript-2 Protein
• T Cell-Interacting, Activating Receptor On Myeloid Cells 1
• T Cell-Interacting, Activating Receptor On Myeloid Cells
• TARM1
• T-Cell-Interacting, Activating Receptor On Myeloid Cells Protein 1

Background

TARM-1 (T cell interacting, activating receptor on myeloid cells-1), also name OLT-2 (OSCAR-like Transcript 2), is a type 1 membrane protein expressed by neutrophils, inflammatory monocytes, macrophages, and dendritic cells. Mature mouse TARM-1 contains a 242 amino acid (aa) long extracellular domain with two Ig-like C2 domains, a 21 aa long transmembrane domain and a 9 aa long cytoplasmic domain. The extracellular domain of mouse TARM-1 shares 81.6% and 46.5% aa identity with rat and human TARM-1 respectively. TARM-1 associates with the ITAM bearing adaptor FcR gamma , but not with DAP10 or DAP12. TARM1 expression is also up-regulated by bone marrow-derived macrophages and dendritic cells following stimulation with TLR agonists in vitro. TARM1 receptor stimulation on macrophages and neutrophils co-stimulate the secretion of proinflammatory cytokines induced by TLR ligands, such as LPS. TARM1 Fc fusion protein inhibits anti-CD3 induced CD4⁺ cell activation, suggesting that TARM1 ectodomain interacts with an unidentified receptor on T cells to inhibit T cell activation (1).

1. Radjabova, V. et al. (2015) J Immunol. 195:3149.

Publications for OLT-2 (10000-TA)(1)

Publication using 10000-TA

• Huang, XR;Ye, L;An, N;Wu, CY;Wu, HL;Li, HY;Huang, YH;Ye, QR;Liu, MD;Yang, LW;Liu, JX;Tang, JX;Pan, QJ;Wang, P;Sun, L;Xia, Y;Lan, HY;Yang, C;Liu, HF; Macrophage autophagy protects against acute kidney injury by inhibiting renal inflammation through the degradation of TARM1 Autophagy 2024-08-28 [PMID: 39193910] (Bioassay, Human, Mouse)

Bioinformatics

• Uniprot:
  • B6A8R8()