Recombinant Mouse IL-Y Protein, CF

Recombinant Mouse IL-Y (Catalog # 9989-IL) inhibits IL‑12 induced IFN-gamma secretion in mouse splenocytes. The ED50 for this effect is 10‑60 ng/mL.

2 μg/lane of Recombinant Mouse IL-Y was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 74‑84 kDa.

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse IL-Y Protein, CF Summary

• Details of Functionality: Measured by its ability to inhibit IL-12 induced IFN-gamma secretion by mouse splenocytes. The ED₅₀ for this effect is 10-60 ng/mL.
• Source: Mouse myeloma cell line, NS0-derived mouse IL-Y (IL-12p40/IL-27p28) protein
  

  Mouse IL-12p40 (Met23-Ser335) Accession # P43432	GGGSGGGSGGGS	Mouse IL-27p28 (Phe29-Ser234) Accession # Q8K3I6
  N-terminus		C-terminus

• Accession #: P43432, Q8K3I6
• N-terminal Sequence: Met23 (IL-12p40)
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 60 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 74-84 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage:
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Reconstitution Instructions: Reconstitute at 200 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse IL-Y Protein, CF

• IL-Y (IL-12p40/IL-27p28)

Background

IL-Y is a new member of the IL-12 cytokine family consists of IL-12p40 and IL-27p28 subunits (1, 2, 3). The mature mouse IL-12p40 subunit contains 312 amino acids and shares 66% and 93%amino acid (aa) identity with human and rat IL-12p40, respectively. The mature mouse IL-27p28 subunit contains 205 amino acids and shares 70% and 89% aa identity with human and rat IL-27p28, respectively. IL-Y inhibits the differentiation and inflammatory responses of Th1 and Th17 cells while promoting expansion of IL-10 and Foxp3 expressing regulatory T cells (3). Treatment of prediabetic non-obese diabetic mice using adenovirus vector expressing IL-Y prevents the onset of hyperglycemia with reduced expression of inflammatory mediators such as IFN-gamma . IL-Y significantly stimulates a unique cytokine and chemokine expression profile as well as to activate STAT3, in part, through a pathway involving WSX-1 (2).

1. Hasegawa, H. et al. (2016) Frontiers in Immunology 7:479.
2. Flores, R. et al. (2015) Eur J Immunol. 45:3114.
3. Wang, R. et al. (2012) The Journal of Biological Chemistry 287:36012.

Bioinformatics

• Uniprot:
  • P43432, Q8K3I6()