Recombinant Mouse IL-6/IL-6R alpha Protein Chimera

Images

 
Recombinant Mouse IL-6/IL-6R alpha complex (Catalog #9038-SR) inhibits M1 mouse myeloid leukemia cell proliferation. The ED50 for this effect is 2‑12 ng/mL.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity

Order Details

View Available Formulations
Catalog# & Formulation Size Price

Recombinant Mouse IL-6/IL-6R alpha Protein Chimera Summary

Details of Functionality
Measured by its ability to inhibit M1 mouse myeloid leukemia cell proliferation. The ED50 for this effect is 2‑12 ng/mL.
Source
Mouse myeloma cell line, NS0-derived mouse IL-6/IL-6R alpha Complex protein
Mouse IL-6R alpha
(Leu20-Glu357)
Accession # P22272
GGGSGGGSGGGS Mouse IL-6
(Phe25-Thr211)
Accession # P08505
N-terminusC-terminus
Accession #
N-terminal Sequence
Leu20 (major), Leu28 (minor)
Protein/Peptide Type
Recombinant Proteins
Gene
Il6
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
70-87 kDa, reducing conditions
Publications
Read Publications using
9038-SR in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 200 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse IL-6/IL-6R alpha Protein Chimera

  • IL-6/IL-6R alpha Complex

Background

Interleukin-6 (IL-6) is a pleiotropic, alpha -helical, 22-28 kDa phosphorylated and variably glycosylated cytokine that plays important roles in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression. IL-6, along with TNF-alpha and IL-1, drives the acute inflammatory response and the transition from acute inflammation to either acquired immunity or chronic inflammatory disease (1-5). When dysregulated, it contributes to chronic inflammation in obesity, insulin resistance, inflammatory bowel disease, arthritis, sepsis, and atherosclerosis (1-3). IL-6 can also function as an anti-inflammatory molecule, as in skeletal muscle where it is secreted in response to exercise (2). In addition, it enhances hematopoietic stem cell proliferation and the differentiation of Th17 cells, memory B cells, and plasma cells (1, 6). IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6R alpha) and a signal transducing subunit (gp130). IL-6 binds to IL-6 R alpha , triggering IL-6 R alpha association with gp130 and gp130 dimerization (7). gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM (8). Soluble forms of IL-6 R alpha are generated by both alternative splicing and proteolytic cleavage (3). In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R alpha elicit responses from gp130-expressing cells that lack cell surface IL-6 R alpha (3). Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous, while that of IL-6 R alpha is predominantly restricted to hepatocytes, monocytes, and resting lymphocytes (2, 3). Soluble splice forms of gp130 block trans-signaling from IL-6/IL-6 R alpha but not from other cytokines that use gp130 as a co-receptor (3, 9). Mature mouse IL-6 shares 39% and 85% amino acid (aa) sequence identity with human and rat IL-6, respectively (10-12). Within the extracellular domain, mouse IL-6 R alpha shares 51% and 89% aa sequence identity with human and rat IL-6 R alpha , respectively (13).
  1. Mansell, A. and B.J. Jenkins (2013) Cytokine Growth Factor Rev. 24:249.
  2. Schuett, H. et al. (2009) Thromb. Haemost. 102:215.
  3. Hunter, C.A. and S.A. Jones (2015) Nat. Immunol. 16:448.
  4. Erta, M. et al. (2012) Int. J. Biol. Sci. 8:1254.
  5. Garbers, C. et al. (2012) Cytokine Growth Factor Rev. 23:85.
  6. Cerutti, A. et al. (1998) J. Immunol. 160:2145.
  7. Murakami, M. et al. (1993) Science 260:1808.
  8. Muller-Newen, G. (2003) Sci. STKE 2003:PE40.
  9. Mitsuyama, K. et al. (2006) Clin. Exp. Immunol. 143:125.
  10. Chiu, C.P. et al. (1988) Proc. Natl. Acad. Sci. USA 85:7099.
  11. Simpson, R.J. et al. (1988) Eur. J. Biochem. 176:187.
  12. Van Snick, J. et al. (1988) Eur. J. Immunol. 18:193.
  13. Sugita, T. et al. (1990) J. Exp. Med. 171:2001.

Publications for IL-6/IL-6R alpha Complex (9038-SR)(2)

Reviews for IL-6/IL-6R alpha Complex (9038-SR) (0)

There are no reviews for IL-6/IL-6R alpha Complex (9038-SR). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for IL-6/IL-6R alpha Complex (9038-SR) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional IL-6/IL-6R alpha Complex Products

Bioinformatics Tool for IL-6/IL-6R alpha Complex (9038-SR)

Discover related pathways, diseases and genes to IL-6/IL-6R alpha Complex (9038-SR). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Blogs on IL-6/IL-6R alpha Complex

There are no specific blogs for IL-6/IL-6R alpha Complex, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Mouse IL-6/IL-6R alpha Protein Chimera and receive a gift card or discount.

Bioinformatics