Recombinant Mouse CEACAM-20 His-tag Protein, CF

Immobilized Recombinant Mouse CEACAM-20 His-tag Protein (Catalog # 10953-CM) enhances L cells mouse fibroblast cell line adhesion. The ED50 for this effect is 0.300‑1.50 μg/mL.

2 μg/lane of Recombinant Mouse CEACAM-20 His-tag Protein (Catalog # 10953-CM) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 63-73 kDa.

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse CEACAM-20 His-tag Protein, CF Summary

• Details of Functionality: Measured by the ability of the immobilized protein to support the adhesion of the L Cells mouse fibroblast cell line. The ED₅₀ for this effect is 0.300-1.50 μg/mL.
• Source: Mouse myeloma cell line, NS0-derived mouse CEACAM-20 protein
  His31-Ala430
• Accession #: Q9D2Z1.1
• N-terminal Sequence: His31
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 45 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 63-73 kDa, under reducing conditions.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse CEACAM-20 His-tag Protein, CF

• CEACAM20
• CEACAM-20
• GPAD9366
• UNQ9366

Background

Carcinoembryonic antigen-related cell adhesion molecule-20 (CEACAM-20) is a member of the CEACAM subfamily of glycoproteins in the immunoglobulin (Ig) superfamily primarily found in mammals. Mature mouse CEACAM-20 consists of an extracellular domain (ECD) with a truncated IgV-like N domain, unique among CEACAMs, and four IgC2-like domains, a transmembrane domain, and a cytoplasmic domain (1-5). The cytoplasmic domain is unusually long compared to most other CEACAMs and is predicted to contain four tyrosine phosphorylation sites, two of which correspond to the immune-receptor tyrosine-based activation motif (ITAM) (2, 3). The ECD of mouse CEACAM-20 shares 59% and 82% amino acid sequence identity to human and rat CEACAM-20, respectively. CEACAM proteins, along with the closely related pregnancy-specific glycoproteins (PSGs), have been linked to numerous intercellular-adhesion and intracellular signaling processes including cell adhesion, growth, and recognition, differentiation, angiogenesis, apoptosis as well as pathogen transmission, tumorigenesis, and fetal–maternal interactions (5, 7, 8). CEACAM-20 expression is limited to the reproductive system and the intestinal tract, with the highest levels of expression found in the small intestine and prostate (2, 3). An in vitro model of human prostate morphogenesis showed that CEACAM-20 is co-expressed with CEACAM-1 and plays a critical role in the formation of prostate organoids, making it a marker for prostate cancer (2). Although the exact mechanism is not fully understood, CEACAM-20 may promote the proliferation of intestinal epithelial cells (IECs) (9). There is evidence suggesting CEACAM-20 can induce the production of chemokines like interleukin (IL)-8 and stimulate inflammatory responses in colitis and Crohn's disease (6). CEACAM-20 is also thought be act as a physiological substrate for SAP-1 in the intestinal epithelium (10).

1. Tchoupa, A. et al. (2014) J Cell Commun Signal 12:27.
2. Zhang, H. et al. (2013) PLoS ONE 8:e53359.
3. Zebhauser, R. et al. (2005) Genomics 86:566.
4. Beauchemin, N. Arabzadeh, A. (2013) Cancer Metastasis Rev 32(3):643.
5. Kuespert, K. et al. (2006) Curr Opin Cell Biol 18:565.
6. Murata, Y. et al. (2015) PNAS E4264-4271.
7. Chang, C.L. et al. (2013) PLoS One. 8:e61701.
8. Horst, A.K. and Wagener, C. (2004) Handb Exp Pharmacol 283-341.
9. Kitamura, Y. et al. (2015) Genes to Cells 20:578.
10. Kotani, et al. (2016) Expert Review of Gastroenterology & Hepatology. 10:1313.

Bioinformatics

• Uniprot:
  • Q9D2Z1.1()