Recombinant Monkeypox Virus Zaire-96-I-16 E8L Protein, CF

2 μg/lane of Recombinant Monkeypox Virus Zaire-96-I-16 E8L His-tag Protein (Catalog # 11263-MX) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 40-46 kDa.

• Format: Carrier-Free

Recombinant Monkeypox Virus Zaire-96-I-16 E8L Protein, CF Summary

• Additional Information: His-tag
• Details of Functionality: Bioassay data are not available.
• Source: Human embryonic kidney cell, HEK293-derived monkeypox virus zaire-96-i-16 E8L protein
  Met1-Thr275, with a C-terminal 6-His tag
• Accession #: NP_536532.1
• N-terminal Sequence: Met1
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity not tested
• Theoretical MW: 33 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 40-46 kDa, under reducing conditions.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Monkeypox Virus Zaire-96-I-16 E8L Protein, CF

• E8L
• MPXV-E8L
• OPG120

Background

Monkeypox Virus (MPXV), the virus that causes monkeypox infection in both humans and animals, is a double-stranded DNA virus that has had a recent global outbreak in 2022 (1). MPXV belongs to the Poxviridae family of viruses (2). It consists of several key subunits including a surface membrane fusion protein (A29L, ~14 kDa), two separate envelope proteins (A30L ~14 kDa and H3L ~32kDa), an envelope glycoprotein, A35R ~15 kDa), a receptor glycoprotein that mimics IFN‑alpha/beta (B16, ~37kDa), a palmitoylated EEV membrane glycoprotein (C19L, ~35 kDa), a secreted IL-18 binding protein (D6L, ~14kDa), a cell surface-binding protein (E8L, ~32 kDa), a telomere binding protein (I1L, ~36kDa), and a subunit required for DNA packaging (L1R, 18 kDa) (2-3).The E8L subunit in MPXV is a surface binding protein that plays a key role in viral entry (4). This happens with E8L binding to chondroitin sulfate on the surface of cells, giving MPXV virion attachment to the host cell. In infected samples, E8L is identified in abundance with intracellular proteins (5).  When targeting the E8L subunit, transmission and replication was reduced by 78% when treated with 100 nM of small interference RNA (siRNA) and reduced by 95% when treated with 200 nM of siRNA (4). The E8L subunit is the most promising target for a newer, more effective vaccine against MPXV, as it has been found to contain human-foreign pentapeptides that could contain epitopes that would be viable for MPXV vaccinations (4, 6-7).

1. Breman, J.G. et al. (1980) Bull World Health Organ. 58:165.
2. Farahat, R.A. et al. (2022) Infez Med. 30:372.
3. Schelkunov, S.N. et al. (2002) Virology 297:172.
4. Alkhalil, A. et al. (2009) Virology Journal 6:1.
5. Brown, J.N. et al. (2010) Mol Cell Proteomics 9:2760.
6. Focosi, D. et al. (2022)Reviews in Medical Virology: e2392.
7. Gao, A. et al. (2022) Research Square: DOI: https://doi.org/10.21203/rs.3.rs-1693979/v1.

Bioinformatics

• Uniprot:
  • NP_536532.1()