Recombinant Monkeypox Virus H3L His-tag Protein, CF

Recombinant Monkeypox Virus Zaire-96-I-16 H3L His-tag Protein (Catalog # 11277-MX) has a molecular weight (MW) of 36.4 kDa as analyzed by SEC-MALS, suggesting that this protein is a monomer. MW may differ from predicted MW due to post-translational modifications (PTMs) present (e.g. Glycosylation).

2 μg/lane of Recombinant Monkeypox Virus H3L His-tag Protein (Catalog # 11277-MX) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 36-40 kDa.

• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Monkeypox Virus H3L His-tag Protein, CF Summary

• Additional Information: Analyzed by SEC-MALS
• Details of Functionality: Bioassay data are not available.
• Source: Human embryonic kidney cell, HEK293-derived monkeypox virus zaire-96-i-16 H3L protein
  Met1-Pro278, with a C-terminal 6-His tag
• Accession #: NP_536520.1
• N-terminal Sequence: Met1 & Lys5
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity2
• Theoretical MW: 33 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 36-40 kDa, under reducing conditions.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Monkeypox Virus H3L His-tag Protein, CF

• H3L

Background

Monkeypox Virus (MPXV), the virus that causes monkeypox infection in both humans and animals, is a double-stranded DNA virus that has had a recent global outbreak in 2022 (1). MPXV belongs to the Poxviridae family of viruses (2). It consists of several key subunits including a surface membrane fusion protein (A29L, ~14 kDa), two separate envelope proteins (A30L ~14 kDa and H3L ~32kDa), an envelope glycoprotein, A35R ~15 kDa), a receptor glycoprotein that mimics IFN‑alpha/beta (B16, ~37 kDa), a palmitoylated EEV membrane glycoprotein (C19L, ~35 kDa), a secreted IL-18 binding protein (D6L, ~14 kDa), a cell surface-binding protein (E8L, ~32 kDa), a telomere binding protein (I1L, ~36kDa), and a subunit required for DNA packaging (L1R, 18 kDa) (2, 3). The H3L subunit is an envelope protein that assists in virion attachment (4). It belongs to the chordopoxvirinae protein H3 family and is 278 ammino acids long (2). H3L has been looked at for use in testing kits and immunoassays due to its high abundance in MPXV patients after viral infection (5). Other studies have shown that the H3L subunit plays a role in the Vaccinia virus based vaccine's ability to cross vaccinate from MPXV (6).

1. Breman, J.G. et al. (1980) Bull World Health Organ. 58:165.
2. Farahat, R.A. et al. (2022) Infez Med. 30:372.
3. Schelkunov, S.N. et al. (2002) Virology 297:172.
4. Nicolas, L. et al. (2022) Travel. Med. Infect. Dis. 49:102402.
5. Yefet, R. et al. (2022) medRxiv doi: https://doi.org/10.1101/2022.08.22.22278946.
6. Ahmed, S.F. et al. (2022) Viruses 14:1960.

Bioinformatics

• Uniprot:
  • NP_536520.1()