Recombinant Monkeypox Virus A30L His-tag Protein, CF

Recombinant Monkeypox Virus Zaire-96-I-16 A30L His-tag Protein (Catalog # 11282-MX) has a molecular weight (MW) of 20.1 kDa as analyzed by SEC-MALS, suggesting that this protein is a monomer.

2 μg/lane of Recombinant Monkeypox Virus A30L His-tag Protein (Catalog # 11282-MX) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 22-26 kDa.

• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Monkeypox Virus A30L His-tag Protein, CF Summary

• Additional Information: Analyzed by SEC-MALS
• Details of Functionality: Bioassay data are not available.
• Source: Human embryonic kidney cell, HEK293-derived monkeypox virus zaire-96-i-16 A30L protein
  Gln22-Leu146, with a C-terminal 6-His tag
• Accession #: NP_536567.1
• N-terminal Sequence: Gln22 inferred from enzymatic pyroglutamate treatment revealing Ser23
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 15 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 22-26 kDa, under reducing conditions.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Monkeypox Virus A30L His-tag Protein, CF

• A30L

Background

Monkeypox Virus (MPXV), the virus that causes monkeypox infection in both humans and animals, is a double-stranded DNA virus that has had a recent global outbreak in 2022 (1). MPXV belongs to the Poxviridae family of viruses (2). It consists of several key subunits including a surface membrane fusion protein (A29L, ~14 kDa), two separate envelope proteins (A30L ~14 kDa and H3L ~32 kDa), an envelope glycoprotein, A35R ~15 kDa), a receptor glycoprotein that mimics IFN‑alpha/beta (B16, ~37 kDa), a palmitoylated EEV membrane glycoprotein (C19L, ~35 kDa), a secreted IL-18 binding protein (D6L, ~14 kDa), a cell surface-binding protein (E8L, ~32 kDa), a telomere binding protein (I1L, ~36 kDa), and a subunit required for DNA packaging (L1R, 18 kDa) (2, 3). The A30L subunit is 146 amino acids long and one of the two envelope proteins responsible for allowing for viral entry into a host cell and starting cell to cell fusion (4). The A30L subunit has been the focus of multiple studies for vaccination purposes for not only monkeypox, but for smallpox as well (4). The A30L subunit has also been looked into for development in detection kits for MPXV (5).

1. Breman, J.G. et al. (1980) Bull World Health Organ. 58:165.
2. Farahat, R.A. et al. (2022) Infez Med. 30:372.
3. Schelkunov, S.N. et al. (2002) Virology 297:172.
4. Rinat, A.M. et al. (2013) Journal of Vaccines http://dx.doi.org/10.1155/2013/618324.
5. Dumont, C. et al. (2014) PLOS One 9:e96930.

Bioinformatics

• Uniprot:
  • NP_536567.1()