Recombinant MERS-CoV His6 Papain-like Protease Protein, CF

• Reactivity: V
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant MERS-CoV His6 Papain-like Protease Protein, CF Summary

• Details of Functionality: Reaction conditions will need to be optimized for each specific application. We recommend an initial PLPro concentration of 20-100 nM when using Ubiquitin-Rh110 (U-555) substrate and 100-500 nM when using tetraubiquitin substrate.
• Source: E. coli-derived mers-cov Papain-like Protease protein
  Leu1541 - Ser1859 with a N-terminal 6-His tag
• Accession #: K9N638.1
• Protein/Peptide Type: Recombinant Enzymes
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 37 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
• Buffer: Supplied as a solution in HEPES, NaCl, DTT and Glycerol.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant MERS-CoV His6 Papain-like Protease Protein, CF

• Papain-like Protease
• PLpro
• PL-PRO
• pp1a
• Replicase polyprotein 1a

Background

The Papain-like protease ("PLPro") from the human Middle East Respiratory Syndrome (MERS) virus is essential for viral replication.  In addition to cleaving the replicase polyprotein at sites located at the N-terminus of the replicase polyprotein, PLPro possesses both deubiquitinating and deISGylating activity and is capable of hydrolyzing polyubiquitin chains.  PLPro antagonizes the induction of type I interferon signaling by clocking the phosphorylation and subsequent nuclear translocation of IRF3; it also prevents host NF kappa B signaling.  MERS PLPro will hydrolyze K6-, K11-, K33-, K48- and K63-linked tetraubiquitin chains in vitro, and will also hydrolyze Ubiquitin-Rh110.  

1. Clasman J.R. et al. (2020) Antiviral Res. 174:104661.

Publications for Papain-like Protease (E-609)(1)

Publication using E-609

• Mali, SS;Silva, R;Gong, Z;Cronce, M;Vo, U;Vuong, C;Moayedi, Y;Cox, JS;Bautista, DM; SARS-CoV-2 papain-like protease activates nociceptors to drive sneeze and pain bioRxiv : the preprint server for biology 2024-01-11 [PMID: 38260476] (Bioassay, Human)

Bioinformatics

• Uniprot:
  • K9N638.1()