Recombinant MERS-CoV His6 Papain-like Protease Protein, CF

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Product Details

Summary
Reactivity VSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant MERS-CoV His6 Papain-like Protease Protein, CF Summary

Details of Functionality
Recombinant MERS virus PLPro is a Ubiquitin- and ISG15-deconjugating enzyme.  Reaction conditions will need to be optimized for each specific application.  We recommend an initial PLPro concentration of 20-100 nM when using Ubiquitin-Rh110 (U-555) substrate and 100-500 nM when using tetraubiquitin substrate.
Source
E. coli-derived mers-cov Papain-like Protease protein
Leu1541 - Ser1859
Accession #
Protein/Peptide Type
Recombinant Enzymes
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Applications/Dilutions

Theoretical MW
37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 100 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Buffer
X mg/ml (X  μM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% (v/v) Glycerol, 1 mM DTT
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant MERS-CoV His6 Papain-like Protease Protein, CF

  • Papain-like Protease
  • PLpro
  • PL-PRO
  • pp1a
  • Replicase polyprotein 1a

Background

The Papain-like protease ("PLPro") from the human Middle East Respiratory Syndrome (MERS) virus is essential for viral replication.  In addition to cleaving the replicase polyprotein at sites located at the N-terminus of the replicase polyprotein, PLPro possesses both deubiquitinating and deISGylating activity and is capable of hydrolyzing polyubiquitin chains.  PLPro antagonizes the induction of type I interferon signaling by clocking the phosphorylation and subsequent nuclear translocation of IRF3; it also prevents host NF kappa B signaling.  MERS PLPro will hydrolyze K6-, K11-, K33-, K48- and K63-linked tetraubiquitin chains in vitro, and will also hydrolyze Ubiquitin-Rh110.  This recombinant protein contains an N-terminal 6-His tag.
  1. Clasman J.R. et al. (2020) Antiviral Res.  doi: 10.1016/j.antiviral.2019.104661

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