Recombinant Influenza A Virus H1N1 Hemagglutinin Protein, CF

When Recombinant Human Galectin-1 (1152-GA) immobilized at 10.00 μg/mL, 100 μL/well, the concentration of Recombinant Influenza A Virus H1N1 Hemagglutinin His-tag Protein (Catalog # 10973-HA) that produces 50% of the optimal binding response is 0.800-8.00 μg/mL.

2 μg/lane of Recombinant Influenza A Virus H1N1 Hemagglutinin His-tag Protein (Catalog # 10973-HA) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 68-83 kDa.

• Reactivity: V
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Influenza A Virus H1N1 Hemagglutinin Protein, CF Summary

• Additional Information: His-tag (HEK293-Expressed)
• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Human Galectin-1 (Catalog # 1152-GA) immobilized at 10.00 μg/mL, 100 μL/well, the concentration of Recombinant Influenza A Virus H1N1 His-tag (Catalog # 10973-HA) that produces 50% of the optimal binding response is 0.8-8.0 μg/mL.
• Source: Human embryonic kidney cell, HEK293-derived influenza a virus h1n1 Hemagglutinin protein
  Asp18-Gln529, with a C-terminal 6-His tag
• Accession #: YP_009118626.1
• N-terminal Sequence: Asp18
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 58 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 68-83 kDa, under reducing conditions.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Influenza A Virus H1N1 Hemagglutinin Protein, CF

• HA
• Hemagglutinin

Background

Hemagglutinin (HA) and neuraminidase (NA) are major membrane glycoproteins found on the surface of influenza virus. The two proteins are essential for the infectious cycle of the influenza virus. During initial infection, HA binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. HA exists as a homotrimer, with each monomer composed of a globular HA1 domain and the stalk-like HA2 domain. HA has evolved to recognize long alpha 2-6 sialylated glycans for efficient transmission in human population.  Although the HA sequence patterns of the 1918 pandemic and 2009 pandemic viruses are similar, they were caused by different H1N1 subtype viruses. The H1N1 1918 HA shares 86% amino acid sequence identity with the H1N1 2009 HA. Inhibiting HA-mediated membrane fusion is a target for influenza therapies. The HA section in the 1918 virus is being currently used to investigate how the 1918 pandemic spread and how a modern pandemic influenza virus would play out in the future.

1. Michal Lazniewski, M. et al. (2018) Briefings in Functional Genomics 17:415.
2. Sriwilaijaroen, N. and Suzuki, Y. (2012) Proc Jpn Acad Ser B Phys Biol Sci. 88:226.
3. Chang, Y.J. et al. (2021) Sci Rep 11:8637.
4. Short, K. et al. (2018) Front. Cell. Infect. Microbiol. 8:343.

Bioinformatics

• Uniprot:
  • YP_009118626.1()