Recombinant Human VSTM2L HA-tag Protein, CF

Recombinant Human VSTM2L (Catalog # 2111-VT) inhibits IFN-gamma secretion by humanperipheral blood mononuclear cells in the presence of anti-CD3 antibody. TheED50 for this effect is 0.6-6 μg/mL.

2 μg/lane of Recombinant Human VSTM2L was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 21-28 kDa.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human VSTM2L HA-tag Protein, CF Summary

• Details of Functionality: Measured by its ability to inhibit anti-CD3 antibody induced IFN-gamma secretion by human peripheral blood mononuclear cells (PBMC). The ED₅₀ for this effect is 0.6-6 μg/mL.
• Source: Chinese Hamster Ovary cell line, CHO-derived human VSTM2L protein
  

  Hemagglutinin Tag (YPYDVPDYA)	Human VSTM2L (Thr25-Leu204) Accession # Q96N03
  N-terminus	C-terminus

• Accession #: Q96N03
• N-terminal Sequence: HA-tag followed by Thr25
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 21 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 21-28 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage:
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Reconstitution Instructions: Reconstitute at 200 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human VSTM2L HA-tag Protein, CF

• C20orf102
• chromosome 20 open reading frame 102
• dJ1118M15.2
• Gm691
• V-set and transmembrane domain containing 2 like
• V-set and transmembrane domain-containing protein 2-like protein
• VSTM2L

Background

V-set and transmembrane domain-containing protein 2-like protein (VSTM2L), also known as C20 or f102, is a 22 kDa extracellular protein belonging to the Immunoglobulin superfamily. VSTM2L contains an N-terminal signal peptide (aa 1-25), and an Ig-like domain (aa 41-158) with a disulfide bond between Cys62 and Cys142. VSTM2L is expressed primarily in the central nervous system, but is also detected in serum and cultured cells (1). It has been found to be an antagonist of the neuroprotective peptide Humanin (1). Human VSTM2L has a 94% sequence identity to rat and mouse homolog. VSTM2L functions as an inhibitory receptor in macrophages (2). Our in house data show that VSTM2L inhibits the human T cell activation, including anti-CD3 induced IL-2 and IFN-gamma secretion, and T cell proliferation.

1. Rossini L. et al. (2011) FASEB J. 25:1983.
2. Yu, Z. et al. (2001) J. Biol. Chem. 276: 23816.

Bioinformatics

• Uniprot:
  • Q96N03()