Recombinant Human VEGF/PlGF Heterodimer Protein, CF

Product Discontinued

Recombinant Human VEGF/PlGF Heterodimer Protein, CF Summary

• Details of Functionality: Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. Conn, G. et al. (1990) Proc. Natl. Acad. Sci. USA 87:1323. The ED₅₀ for this effect is 15‑75 ng/mL.
• Source: E. coli-derived human VEGF/PlGF Heterodimer protein
  

  Human VEGF (Ala27-Arg191) Accession # AAM03108

  Human PlGF (Ala21-Arg149) Accession # AAD30179
  N-terminus	C-terminus

• Accession #: AAM03108 (VEGF) & AAD30179 (PlGF)
• N-terminal Sequence: Ala27 (VEGF) & Ala21 (PlGF)
• Structure / Form: Disulfide-linked heterodimer
• Protein/Peptide Type: Recombinant Proteins
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 19.2 kDa (VEGF) and 14.5 kDa (PIGF).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human VEGF/PlGF Heterodimer Protein, CF

• VEGF/PlGF Heterodimer

Background

The disulfide-linked homodimeric proteins vascular endothelial growth factor (VEGF) and placenta growth factor (PlGF) are both members of the VEGF family of growth factors. VEGF and PlGF proteins share primary structural as well as limited amino acid sequence homology with the A and B chains of PDGF. All eight cysteine residues involved in intra- and inter-chain disulfides are conserved among these growth factors. In their PDGF-like regions, VEGF and PlGF also share approximately 53% amino acid sequence similarity. E. coli-expressed monomers of PlGF and VEGF can be refolded in vitro to form PlGF/VEGF heterodimers. The purified recombinant VEGF/PlGF heterodimers and VEGF homodimers have potent mitogenic effects on endothelial cells. However, VEGF/PlGF heterodimers display approximately 50-fold less mitogenic activity than VEGF homodimers. In contrast, PlGF homodimers have little or no mitogenic effect on endothelial cells. The presence of natural VEGF/PlGF heterodimers in the conditioned media of a number of human tumor cell lines has been demonstrated using a VEGF/PlGF heterodimer specific ELISA.

Publications for VEGF/PlGF Heterodimer (297-VP/CF)(4)

Publications using 297-VP/CF

• I Apicella, V Cicatiello, D Acampora, V Tarallo, S De Falco Full Functional Knockout of Placental Growth Factor by Knockin with an Inactive Variant Able to Heterodimerize with VEGF-A Cell Rep, 2018-06-19;23(12):3635-3646. 2018-06-19 [PMID: 29925004] (In Vivo, Mouse)
• Brave SR, Eberlein C, Shibuya M, Wedge SR, Barry ST Placental growth factor neutralising antibodies give limited anti-angiogenic effects in an in vitro organotypic angiogenesis model. Angiogenesis, 2010-10-16;13(4):337-47. 2010-10-16 [PMID: 20953695] (Bioassay, Human)
• Sun JF, Phung T, Shiojima I, Felske T, Upalakalin JN, Feng D, Kornaga T, Dor T, Dvorak AM, Walsh K, Benjamin LE Microvascular patterning is controlled by fine-tuning the Akt signal. Proc. Natl. Acad. Sci. U.S.A., 2004-12-20;102(1):128-33. 2004-12-20 [PMID: 15611473] (Bioassay, Bovine)
• Oura H, Bertoncini J, Velasco P, Brown LF, Carmeliet P, Detmar M A critical role of placental growth factor in the induction of inflammation and edema formation. Blood, 2002-09-05;101(2):560-7. 2002-09-05 [PMID: 12393422] (In Vivo, Mouse)

Bioinformatics

• Uniprot:
  • AAM03108 (VEGF) & AAD30179 (PlGF)()