Recombinant Human TLR4/MD-2 Complex Protein, CF

Images

 
There are currently no images for TLR4/MD-2 Complex (3146-TM/CF).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

View Available Formulations
Catalog# & Formulation Size Price

Recombinant Human TLR4/MD-2 Complex Protein, CF Summary

Details of Functionality
Measured by its ability to inhibit LPS-induced TNF-alpha secretion by PMA-differentiated U937 human histiocytic lymphoma cells. 20 µg/mL of recombinant human TLR4/MD-2 will inhibit >60% of the TNF-alpha secretion induced by LPS.
Source
Mouse myeloma cell line, NS0-derived human TLR4/MD2 Complex protein
Human TLR4
(Glu24-Lys631)
Accession # O00206
10-His tag
Human MD-2
(Glu17-Asn160)
Accession # Q9Y6Y9
10-His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Glu24 (TLR4) and Glu17 (MD-2)
Protein/Peptide Type
Recombinant Proteins
Gene
TLR4
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
70.6 kDa (TLR4), 19.2 kDa (MD-2).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
95 kDa and 34 kDa, reducing conditions
Publications
Read Publications using
3146-TM/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human TLR4/MD-2 Complex Protein, CF

  • TLR4/MD2 Complex
  • TLR4/MD-2 Complex

Background

TLR4 is a 100 kDa type I transmembrane glycoprotein that belongs to the mammalian Toll-Like Receptor family of pathogen pattern recognition molecules. MD‑2, also known as ESOP-1, is a 25 kDa secreted protein that is required for TLR4‑mediated responses to bacterial lipopolysaccharide (LPS) (1 - 3). The human TLR4 cDNA encodes an 839 amino acid (aa) precursor that contains a 23 aa signal sequence, a 608 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 187 aa cytoplasmic domain. TLR4 contains 21 leucine rich repeats in its ECD and one cytoplasmic Toll/IL-1 receptor (TIR) domain (4). The ECD of human TLR4 shares approximately 25% aa sequence identity with other TLRs and 60% - 74% aa sequence identity with bovine, equine, feline, mouse, rat, and porcine TLR4. The human MD‑2 cDNA encodes a 160 aa precursor with an 18 aa signal sequence (5). Human MD‑2 shares 20% aa sequence identity with MD‑1 and 62% - 64% aa sequence identity with bovine, mouse, and rat MD‑2. MD‑2 associates with TLR4 on monocytes, macrophages, dendritic cells, and B cells (5 - 7). MD2 expression is required for cell surface localization of TLR4 and for optimal LPS‑induced TLR4 signaling (7, 8). MD‑2 also forms soluble disulfide-linked homo-oligomers which can interact with TLR4 (6). Through a domain separate from its TLR4-binding domain, MD‑2 extracts LPS from circulating CD14‑LPS complexes and carries the LPS into a ternary complex with TLR4 (9 - 11). The interaction of MD‑2/LPS with TLR4 induces receptor oligomerization and the triggering of an inflammatory response (12). Increased levels of plasma MD‑2 in septic shock patients sensitizes MD‑2 non-expressing epithelial cells to LPS and promotes widespread tissue inflammation (13).

  1. Gangloff, M. and N.J. Gay (2004) Trends Biochem. Sci. 29:294.
  2. Palsson-McDermott, E.M. and L.A. O’Neill (2004) Immunology 113:153.
  3. Miyake, K. (2004) Semin. Immunol. 16:11.
  4. Medzhitov, R. et al. (1997) Nature 388:394.
  5. Shimazu, R. et al. (1999) J. Exp. Med. 189:1777.
  6. Visintin, A. et al. (2001) Proc. Natl. Acad. Sci. USA 98:12156.
  7. Akashi, S. et al. (2000) J. Immunol. 164:3471.
  8. Nagai, Y. et al. (2002) Nat. Immunol. 3:667.
  9. Re, F. and J.L. Strominger (2003) J. Immunol. 171:5272.
  10. Kennedy, M.N. et al. (2004) J. Biol. Chem. 279:34698.
  11. Gioannini, T.L. et al. (2004) Proc. Natl. Acad. Sci. USA 101:4186.
  12. Saitoh, S. et al. (2004) J. Endotoxin Res. 10:257.
  13. Pugin, J. et al. (2004) Blood 104:4071.

Publications for TLR4/MD-2 Complex (3146-TM/CF)(13)

We have publications tested in 3 confirmed species: Human, Bacteria, N/A.

We have publications tested in 3 applications: Bioassay, Surface Plasmon Resonance, WB Ctrl.


Filter By Application
Bioassay
(8)
Surface Plasmon Resonance
(3)
WB Ctrl
(1)
All Applications
Filter By Species
Human
(6)
Bacteria
(1)
N/A
(3)
All Species
Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using 3146-TM/CF Applications Species
T Vogl, A Stratis, V Wixler, T Völler, S Thurainaya, SK Jorch, S Zenker, A Dreiling, D Chakrabort, M Fröhling, P Paruzel, C Wehmeyer, S Hermann, O Papantonop, C Geyer, K Loser, M Schäfers, S Ludwig, M Stoll, T Leanderson, JL Schultze, S König, T Pap, J Roth Autoinhibitory regulation of S100A8/S100A9 alarmin activity locally restricts sterile inflammation J. Clin. Invest., 2018;0(0):. 2018 [PMID: 29611822] (Bioassay, Human) Bioassay Human
S Sun, M He, Y Wang, H Yang, Y Al-Abed Folic acid derived-P5779 mimetics regulate DAMP-mediated inflammation through disruption of HMGB1:TLR4:MD-2 axes PLoS ONE, 2018;13(2):e0193028. 2018 [PMID: 29447234] (Bioassay) Bioassay
J Honegr, R Dolezal, D Malinak, M Benkova, O Soukup, JSFD Almeida, TCC Franca, K Kuca, R Prymula Rational Design of a New Class of Toll-Like Receptor 4 (TLR4) Tryptamine Related Agonists by Means of the Structure- and Ligand-Based Virtual Screening for Vaccine Adjuvant Discovery Molecules, 2018;23(1):. 2018 [PMID: 29300367] (Bioassay) Bioassay
Westman J, Papareddy P, Dahlgren M, Chakrakodi B, Norrby-Teglund A, Smeds E, Linder A, Morgelin M, Johansson-Lindbom B, Egesten A, Herwald H Extracellular Histones Induce Chemokine Production in Whole Blood Ex Vivo and Leukocyte Recruitment In Vivo. PLoS Pathog, 2015;11(12):e1005319. 2015 [PMID: 26646682] (Surface Plasmon Resonance, N/A) Surface Plasmon Resonance N/A
Loyau J, Malinge P, Daubeuf B, Shang L, Elson G, Kosco-Vilbois M, Fischer N, Rousseau F Maximizing the potency of an anti-TLR4 monoclonal antibody by exploiting proximity to Fcgamma receptors. MAbs, 2014;6(6):1621-30. 2014 [PMID: 25484053] (Surface Plasmon Resonance, Human) Surface Plasmon Resonance Human
Jung I, Shin S, Lee M, Kang T, Han H, Lee S, Kim W, Kim H, Park W, Kim H, Yun C, Lee E, Wu T, Park Y Enhancement of tumor-specific T cell-mediated immunity in dendritic cell-based vaccines by Mycobacterium tuberculosis heat shock protein X. J Immunol, 2014;193(3):1233-45. 2014 [PMID: 24990079] (Bioassay, N/A) Bioassay N/A
Shang L, Daubeuf B, Triantafilou M, Olden R, Depis F, Raby A, Herren S, Dos Santos A, Malinge P, Dunn-Siegrist I, Benmkaddem S, Geinoz A, Magistrelli G, Rousseau F, Buatois V, Salgado-Pires S, Reith W, Monteiro R, Pugin J, Leger O, Ferlin W, Kosco-Vilbois M, Triantafilou K, Elson G Selective antibody intervention of Toll-like receptor 4 activation through Fc gamma receptor tethering. J Biol Chem, 2014;289(22):15309-18. 2014 [PMID: 24737331] (Bioassay, Human) Bioassay Human
Chen, Yu-Fon, Shiau, Ai-Li, Wang, Sheng-Hu, Yang, Jai-Sing, Chang, Sue-Joan, Wu, Chao-Lia, Wu, Tian-Shu Zhankuic acid A isolated from Taiwanofungus camphoratus is a novel selective TLR4/MD-2 antagonist with anti-inflammatory properties. J Immunol, 2014;192(6):2778-86. 2014 [PMID: 24532584]
Fetuin-A acts as an endogenous ligand of TLR4 to promote lipid-induced insulin resistance. Nat Med, 2012;18(0):1279. 2012 [PMID: 22842477] (Bioassay, Human) Bioassay Human
Feng C, Stamatos NM, Dragan AI, Medvedev A, Whitford M, Zhang L, Song C, Rallabhandi P, Cole L, Nhu QM, Vogel SN, Geddes CD, Cross AS Sialyl residues modulate LPS-mediated signaling through the Toll-like receptor 4 complex. PLoS ONE, 2012;7(4):e32359. 2012 [PMID: 22496731] (Bioassay, Human) Bioassay Human
Show All 13 Publications.

Reviews for TLR4/MD-2 Complex (3146-TM/CF) (0)

There are no reviews for TLR4/MD-2 Complex (3146-TM/CF). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

FAQs for TLR4/MD-2 Complex (3146-TM/CF) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Array Products

Bioinformatics Tool for TLR4/MD-2 Complex (3146-TM/CF)

Discover related pathways, diseases and genes to TLR4/MD-2 Complex (3146-TM/CF). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Blogs on TLR4/MD-2 Complex

There are no specific blogs for TLR4/MD-2 Complex, but you can read our latest blog posts.

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human TLR4/MD-2 Complex Protein, CF and receive a gift card or discount.

Bioinformatics

Gene Symbol TLR4
Entrez
Uniprot