Recombinant Human TGF-beta 1.2 Protein

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human TGF-beta 1.2 Protein Summary

Details of Functionality
Measured by its ability to inhibit the IL-4-dependent proliferation of HT‑2 mouse T cells. Tsang, M. et al. (1995) Cytokine 7:389. The ED50 for this effect is 0.04-0.08 ng/mL.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human TGF-beta 1.2 protein
Human TGF-beta 1
(Ala279 - Ser390)
Accession # P01137
Human TGF-beta 2
(Ala303 - Ser414)
Accession # P61812
N-terminus C-terminus
Accession #
N-terminal Sequence
Ala279 (TGF-beta 1) & Ala303 (TGF-beta 2)
Structure / Form
Disulfide-linked heterodimer
Protein/Peptide Type
Recombinant Proteins
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
12.8 kDa (TGF-beta 1) & 12.7 kDa (TGF-beta 2).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
304-B3 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 10 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human TGF-beta 1.2 Protein

  • TGFbeta 1.2
  • TGF-beta 1.2

Background

Transforming Growth Factor Beta (TGF-beta ) is a stable, multifunctional polypeptide growth factor. While specific receptors for this protein have been found on almost all mammalian cell types thus far examined, the effect of the molecule varies depending on the cell type and growth conditions. Generally, TGF-beta is stimulatory for cells of mesenchymal origin and inhibitory for cells of epithelial or neuroectodermal origin. It is now known that the originally described form of TGF-beta, now described as TGF-beta 1, is only one of a family of regulatory proteins consisting of a number of proteins distantly related to TGF-beta 1 (30 - 40% sequence homology) and a number of more closely related proteins (70 - 80% sequence homology) designated TGF-beta 2, TGF-beta 1.2, TGF-beta 3, TGF-beta 4, and TGF-beta 5. TGF-beta 1 has been found in the highest concentration in human platelets and mammalian bone, but is produced by many cell types in smaller amounts. TGF-beta 2 has been found in the highest concentration in porcine platelets and mammalian bone, but again is also produced by many types of cells. The heterodimer, TGF-beta 1.2, has so far been found only in small amounts in porcine platelets. TGF-beta 3 has been detected in human, porcine, and avian sources, mainly in cells of mesenchymal origin, suggesting a different role for this protein than for TGF-beta 1 or -beta 2. TGF-beta 4 has been detected in chick embryo chondrocytes, and its distribution in other types of cells is being investigated. TGF-beta 5 has been detected only in Xenopus embryos. TGF-beta 1, TGF-beta 2, and TGF-beta 1.2 appear to be largely equivalent in biological activity, although there appear to be differences in binding to certain types of receptors, and there are a few reports of differential responses to TGF-beta 1 and TGF-beta 2.

Publications for TGF-beta 1.2 (304-B3)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Bioassay.


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Bioinformatics