Recombinant Human SIRP delta Fc Chimera Protein, CF

When Recombinant Human SIRP delta Fc Chimera (Catalog #10138-SB) is immobilized at 1 µg/mL, recombinant human DIRAS2 binds with anED50 of 0.15-0.9 μg/mL.

2 μg/lane of Recombinant Human SIRP delta Fc Chimera (Catalog# 10138-SB) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR)conditions and visualized by Coomassie® Blue staining, showing bands at 50-60 kDaand 100-120 kDa, respectively.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human SIRP delta Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Human SIRP delta Fc Chimera (Catalog # 10138-SB) is immobilized at 1 µg/mL (100 µL/well), recombinant human DIRAS2 binds with an ED₅₀ of 0.15-0.9 μg/mL.
• Source: Human embryonic kidney cell, HEK293-derived human SIRP delta protein
  

  Human SIRP delta (Phe30-Arg197) Accession # Q9H106	IEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #: Q9H106
• N-terminal Sequence: Phe30
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 45 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 50-60 kDa, under reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human SIRP delta Fc Chimera Protein, CF

• dJ576H24.4
• Protein tyrosine phosphatase non-receptor type substrate 1-like 2
• protein tyrosine phosphatase, non-receptor type substrate 1-like 2
• PTPNS1L2
• signal-regulatory protein delta
• SIRP delta
• SIRPD
• SIRP-Delta

Background

SIRPD (Signal Regulatory Protein Delta), also know as Protein Tyrosine Phosphatase non-Receptor Type Substrate 1-l Like 2 (PTPNS1L2), is member of the signal regulatory proteins (SIRPS) family (1). SIRPD contains a 168 amino acid Ig-like domain that is characteristic of other SIRP family members (1). Unlike other members of SIRPS family, SIRPD lacks the transmemberane region, and is secreted (2). Murine homologs of SIRPD are not characterized. Expression sequence tag analysis suggests that SIRPD may be expressed in sperm cells and respiratory tissue (2). Using BioPlex 2.0 (Biophysical Interactions of ORFeome-derived complexes) high-throughput affinity purification–mass spectrometry (AP–MS) analysis to identify probable protein–protein interactions, several candidate SIRPD interactions were found including DIRAS2 (3). In-house testing indicates SIRPD can interact with DIRAS2.

1. Van den Berg, T.K. et al. (2005) J. Immunol. 175:7788.
2. Van Beek, E.M. et al. (2005) J Immunol. 175:7781.
3. Huttlin, E.L. et al. (2017) Nature 545:505.

Bioinformatics

• Uniprot:
  • Q9H106()