Recombinant Human OLT-2/TARM1 Fc Chimera Protein, CF

Recombinant Human OLT-2/TARM1 Fc Chimera (Catalog # 10059-TA) inhibits IFN gamma secretion by human peripheral blood mononuclear cells in the presence of anti-CD3 antibody. The ED50 for this effect is 2-10 μg/mL.

2 μg/lane of Recombinant Human OLT-2/TARM1 Fc Chimera was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 64-74 kDa and 130- 150 kDa, respectively.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human OLT-2/TARM1 Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its ability to inhibit anti-CD3 antibody induced IFN-gamma secretion by human peripheral blood mononuclear cells (PBMC). The ED₅₀ for this effect is 2-10 μg/mL.
• Source: Chinese Hamster Ovary cell line, CHO-derived human OLT-2/TARM1 protein
  

  Human OLT-2/TARM1 (Gln17-Val235) Accession # NP_001129158.2	IEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #: NP_001129158.2
• N-terminal Sequence: No results obtained. Gln17 inferred from enzymatic pyroglutamate treatment revealing Gly18.
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 50 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 64-74 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human OLT-2/TARM1 Fc Chimera Protein, CF

• OLT2
• OLT-2/TARM1
• OSCAR-Like Transcript-2 Protein
• T Cell-Interacting, Activating Receptor On Myeloid Cells 1
• T Cell-Interacting, Activating Receptor On Myeloid Cells
• TARM1
• T-Cell-Interacting, Activating Receptor On Myeloid Cells Protein 1

Background

T cell interacting, activating receptor on myeloid cells-1 (TARM-1), also known as OSCAR-like transcript-2 protein (OLT-2) is a type 1 membrane protein expressed by neutrophils, inflammatory monocytes, macrophages, and dendritic cells. Mature mouse OLT-2/TARM-1 contains a 242 aa long extracellular domain with two Ig-like C2 domains, a 21 aa long transmembrane domain and a 9 aa long cytoplasmic domain. The extracellular domain of human OLT-2/TARM-1 shares 48% and 49% amino acid (aa) identity with mouse and rat OLT-2/TARM-1 respectively. OLT-2/TARM-1 associates with the ITAM bearing adaptor FcR gamma , but not with DAP10 or DAP12.OLT-2/TARM1 expression is also up-regulated by bone marrow-derived macrophages and dendritic cells following stimulation with TLR agonists in vitro. OLT-2/TARM-1 receptor stimulation on macrophages and neutrophils co-stimulate the secretion of proinflammatory cytokines induced by TLR ligands, such as LPS. OLT-2/TARM-1 Fc fusion protein inhibits anti-CD3 induced CD4⁺ cell activation, suggesting that OLT-2/TARM-1 ectodomain interacts with an unidentified receptor on T cells to inhibit T cell activation (1).

1. Radjabova, V., et al. (2015) J Immunol. 195:3149.

Bioinformatics

• Uniprot:
  • NP_001129158.2()