Recombinant Human LRIT2 Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human LRIT2 Protein, CF Summary

• Details of Functionality: Measured by the ability of the immobilized protein to support the adhesion of BCE C/D‑1b bovine corneal endothelial cells. The ED₅₀ for this effect is 0.6-3 μg/mL.
• Source: Chinese Hamster Ovary cell line, CHO-derived human LRIT2 protein
  Gln21-His462 (Leu220Phe) with a C-terminal 6-His tag
• Accession #: A6NDA9
• N-terminal Sequence: Gln21
• Protein/Peptide Type: Recombinant Proteins
• Gene: LRIT2
• Purity: >95%, by SDS-PAGE with silver staining
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 49 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 67-81 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE with silver staining
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human LRIT2 Protein, CF

• immunoglobulin-like domain and transmembrane domain-containing protein 2
• leucine rich repeat containing 22
• leucine-rich repeat, immunoglobulin-like and transmembrane domains 2
• leucine-rich repeat-containing protein 22
• LRIT2
• LRRC22
• PAL2

Background

Leucine-rich Repeat, Immunoglobulin-like domain and Transmembrane domain-containing protein 2 (LRIT2), also known as leucine-rich repeat protein 22 (LRRC22), is a 550 amino acid (aa) type I transmembrane protein. Its extracellular domain (ECD) contains one LRRNT, LRRCT, Ig-like, and Fibronectin type-III domain, and four leucine-rich repeats (LRRs). Within the ECD, human LRIT2 shares approximately 75% aa sequence identity with the mouse and rat orthologs. The LRR is a structural motif consisting of 20 to 30 aa repeated 2 to 45 times that forms a hydrophobic horseshoe shape with a parallel beta  sheet on the concave side and helical elements on the convex side (1-4). All LRRs are composed of a variable segment and a highly conserved segment, the latter of which accounts for 11 or 12 residues of the entire LRR motif (1-4). LRR motifs mediate protein-protein interactions in a variety of proteins including tyrosine kinase receptors, hormone receptors, extracellular matrix-binding proteins, cell-adhesion molecules, and bacterial virulence factors (1, 3). Proteins containing LRRs play important roles in many biologically processes such as mammalian and neural development, cell polarization, gene expression, and apoptosis (2).

1. Kobe, B. and J. Deisenhofer (1994) Trends Biochem. Sci. 19:415.
2. Kobe, B. and A.V. Kajava (2001) Curr. Opin. Struct. Biol. 11:725.
3. Enkhbayar, P. et al. (2004) Proteins 54:394.
4. Bella, J. et al. (2008) Cell. Mol. Life Sci. 65:2307.

Bioinformatics

• Gene Symbol: LRIT2
• Uniprot:
  • A6NDA9()