Recombinant Human LH alpha/beta Heterodimer Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human LH alpha/beta Heterodimer Protein, CF Summary

• Details of Functionality: Measured by its ability to induce cAMP accumulation in MLTC‑1 mouse Leydig tumor cells. Rebois, R.V. et al. (1982) J. Cell Biol. 94:70. The ED₅₀ for this effect is 0.075-0.75 ng/mL.
• Source: Chinese Hamster Ovary cell line, CHO-derived human LH alpha/beta Heterodimer protein
  Ala25-Ser116 ( alpha chain) & Ser21-Leu141 ( beta chain)
• Accession #: P01215 ( alpha chain) & P01229 ( beta chain)
• N-terminal Sequence: Ala25 ( alpha chain), Ser21 ( beta chain)
• Structure / Form: Non-covalent heterodimer
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 10 kDa ( alpha chain), 13 kDa ( beta chain).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 15-26 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in sterile PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Reconstitution Instructions: Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human LH alpha/beta Heterodimer Protein, CF

• LH alpha/beta Heterodimer

Background

Luteinizing Hormone (LH) is a 42 kDa heterodimer belonging to the glycoprotein hormone family. It is composed of noncovalently linked glycosylated alpha  and beta  chains. The alpha  subunit (CG alpha ) is also a component of Follicle-Stimulating Hormone (FSH), Thyroid-Stimulating Hormone, and Chorionic Gonadotropin. The unique beta  subunit confers the protein’s specific biological action and is responsible for the interaction with its receptor (1, 2). The approximately 20 kDa human CG alpha subunit shares 73% and 72% amino acid (aa) sequence identity with the mouse and rat orthologs, respectively. The approximately 18 kDa human LH beta subunit shares 71% and 72% aa sequence identity with the mouse and rat orthologs, respectively. Multiple isoforms of LH exist due to differences in the post-translational glycosylation, sialylation, and sulphation modifications of its subunits (3-6). The composition, longevity, and activity of the different LH isoforms vary throughout a woman’s menstrual cycle and reproductive life cycle (7). LH is produced and secreted by the anterior pituitary gland. Its secretion is controlled by Gonadotropin-Releasing Hormone from the hypothalamus; however, LH secretion can also be stimulated by estradiol (7, 8). LH works in concert with FSH to regulate female reproduction; FSH stimulates follicular growth and LH induces ovulation (9). LH also drives formation of the corpus luteum by promoting progesterone production (7). Additionally, LH has been suggested to stimulate the adrenal gland in postmenopausal women to induce secretion of sulfated DHEA, a precursor to androgens (10, 11). In the testis, LH induces Leydig cell production of testosterone (7). Hypersecretion of LH has been shown to occur in women with polycystic ovary syndrome and is associated with an increased risk of infertility and miscarriage (12, 13). Additionally, increased serum LH levels are associated with decreased cognition and have been implicated in the development and progression of Alzheimer’s disease (14, 15).

1. Keutmann, H.T. et al. (1987) Proc. Natl. Acad. Sci. USA 84:2038.
2. Keutmann, H.T. et al. (1992) Mol. Endocrinol. 6:904.
3. Wilson, C.A. et al. (1990) J. Endocrinol. 125:3.
4. Stanton, P.G. et al. (1993) J. Endocrinol. 138:529.
5. Stanton, P.G. et al. (1996) Mol. Cell. Endocrinol. 125:133.
6. Bergendah, M. and J.D. Veldhuis (2001) Hum. Reprod. 16:1058.
7. Choi, J. and J. Smitz (2014) Mol. Cell. Endocrinol. 383:203.
8. Marshall, J.C. et al. (1993) Trans. Am. Clin. Climatol. Assoc. 104:31.
9. Raju, G.A. et al. (2013) J. Hum. Reprod. Sci. 6:227.
10. Alevizaki, M. et al. (2006) Eur. J. Endocrinol. 154:875.
11. Moran, F.M. et al. (2013) Menopause 20:329.
12. Balen, A.H. (1995) J. R. Soc. Med. 88:339P.
13. Blank, S.K. et al. (2006) Hum. Reprod. Update 12:351.
14. Webber, K.M. et al. (2007) Clin. Med. Res. 5:177.
15. Blair, J.A. et al. (2015) Front. Endocrinol. (Lausanne) 6:45.

Publications for LH alpha/beta Heterodimer (8899-LH/CF)(1)

Publication using 8899-LH/CF

• W Marlicz, A Poniewiers, S Rzeszotek, R Bartoszews, K Skonieczna, T Starzy?ska, MZ Ratajczak A novel potential role of pituitary gonadotropins in the pathogenesis of human colorectal cancer PLoS ONE, 2018-03-01;13(3):e0189337. 2018-03-01 [PMID: 29494614] (Bioassay, Human)

Bioinformatics

• Uniprot:
  • P01215 ( alpha chain) & P01229 ( beta chain)()