Recombinant Human IL-37b/IL-1F7b Protein

• Reactivity: Hu
• Applications: Bioactivity

Recombinant Human IL-37b/IL-1F7b Protein Summary

• Details of Functionality: Measured by its ability to enhance IL-18 BP inhibition of IL-18-induced IFN-gamma release by KG‑1 human acute myelogenous leukemia cells. Bufler, P. et al. (2002) PNAS 99:13723. The ED₅₀ for this effect is 2.5-12.5 μg/mL.
• Source: E. coli-derived human IL-37b/IL-1F7b protein
  Val46-Asp218
• Accession #: Q9NZH6
• N-terminal Sequence: Val46
• Protein/Peptide Type: Recombinant Proteins
• Gene: IL37
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 19.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 25 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS and DTT with BSA as a carrier protein.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 200 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-37b/IL-1F7b Protein

• IL37b
• IL-37b

Background

Human Interleukin 1 family member 7 (IL-1F7), also named FIL-1 zeta, IL-37, IL-1H4, IL-1HL and IL-1RP1, is an anti-inflammatory member of the IL-1 cytokine family. IL-1F7 shares approximately 21%, 24%, and 30% aa sequence identity with mature IL-1 alpha , IL-1 beta and IL-1ra, respectively. Mouse IL-1F7 has not been reported, but human IL-1F7 is active on mouse cells. Five isoforms (IL-1F7a through e) are generated through alternative exon usage and have distinct expression profiles (1‑5). Like IL-1 alpha , IL-1 beta and IL-18, all of the IL-1F7 variants lack a typical signal peptide. The longest IL-1F7 transcript is the basis for the product offered here. This product, also referred to as IL-1F7b, or IL-1F7 isoform 1, is normally generated from a 218 amino acid (aa) proprotein that consists of a 45 aa propeptide plus a 173 aa mature region that is cleaved by caspase-1 to generate the 32 kDa mature form (aa 46-218) (6). IL-1F7 is up-regulated by inflammatory stimuli in peripheral blood mononuclear cells (7). Experimental overexpression of IL-1F7b in vivo limits the inflammatory response and protects mice from colitis and LPS-induced shock (7, 8). Both unprocessed and mature IL-1F7b can form homodimers in solution (6, 7). Although IL-1F7b will bind to IL-18 R alpha with low affinity, this has no effect on IL‑18 receptor activity (4, 6, 9). Alternatively, IL-1F7b will also bind to IL-18 BP, generating a complex that interacts with IL-18R beta . This has the effect of attenuating IL‑18 activity via the IL-18 receptor (9). IL-1F7b can also function intracellularly. Following LPS-induced cleavage of its propeptide, IL-1F7b associates with Smad3 and translocates to the nucleus. This results in a reduction of pro-inflammatory cytokine secretion (10).

1. Smith, D.E. et al. (2000) J. Biol Chem 275:1169.
2. Kumar S. et al. (2000) J. Biol Chem 275:10308.
3. Busfield S.J. et al. (2000) Genomics 66:213.
4. Pan, G. et al. (2000) Cytokine 13:1.
5. Boraschi, D. et al. (2011) Eur. Cytokine Netw. 22:127.
6. Kumar S. et al. (2002) Cytokine 18:61.
7. Nold, M.F. et al. (2010) Nat. Immunol. 11:1014.
8. McNamee, E.N. et al. (2011) Proc. Natl. Acad. Sci. USA 108:16711.
9. Bufler, P. et al. (2002) Proc. Natl. Acad. Sci. USA 99:13723.
10. Sharma, S. et al. (2008) J. Immunol. 180:5477.

Publications for IL-37b/IL-1F7b (7585-IL)(9)

Publications using 7585-IL

• I Kolotilin Plant-produced recombinant cytokines IL-37b and IL-38 modulate inflammatory response from stimulated human PBMCs Scientific Reports, 2022-11-14;12(1):19450. 2022-11-14 [PMID: 36376518] (Bioassay, Human)
• K Ueno-Shuto, S Kamei, M Hayashi, A Fukuyama, Y Uchida, N Tokutomi, MA Suico, H Kai, T Shuto A Splice Switch in SIGIRR Causes a Defect of IL-37-Dependent Anti-Inflammatory Activity in Cystic Fibrosis Airway Epithelial Cells International Journal of Molecular Sciences, 2022-07-13;23(14):. 2022-07-13 [PMID: 35887095] (Bioassay, Human)
• C Jia, Y Zhuge, S Zhang, C Ni, L Wang, R Wu, C Niu, Z Wen, X Rong, H Qiu, M Chu IL-37b alleviates endothelial cell apoptosis and inflammation in Kawasaki disease through IL-1R8 pathway Cell Death & Disease, 2021-06-03;12(6):575. 2021-06-03 [PMID: 34083516] (Bioassay, In Vivo, Human, Mouse)
• I Tsilioni, H Pantazopou, P Conti, SE Leeman, TC Theoharide IL-38 inhibits microglial inflammatory mediators and is decreased in amygdala of children with autism spectrum disorder Proc. Natl. Acad. Sci. U.S.A., 2020-06-29;0(0):. 2020-06-29 [PMID: 32601180] (Bioassay, Human)
• I Tsilioni, AB Patel, H Pantazopou, S Berretta, P Conti, SE Leeman, TC Theoharide IL-37 is increased in brains of children with autism spectrum disorder and inhibits human microglia stimulated by neurotensin Proc. Natl. Acad. Sci. U.S.A., 2019-10-07;0(0):. 2019-10-07 [PMID: 31591201] (Cell Culture, Human)
• Interleukin-37 Enhances the Suppressive Activity of Naturally Occurring CD4(+)CD25(+) Regulatory T Cells Sci Rep, 2016-12-12;6(0):38955. 2016-12-12 [PMID: 27941849] (Bioassay, Mouse)
• Cavalli G, Koenders M, Kalabokis V, Kim J, Tan A, Garlanda C, Mantovani A, Dagna L, Joosten L, Dinarello C Treating experimental arthritis with the innate immune inhibitor interleukin-37 reduces joint and systemic inflammation. Rheumatology (Oxford), 2016-08-26;55(12):2220-2229. 2016-08-26 [PMID: 27567100] (In Vivo, Mouse)
• Interleukin-37 expression and its potential role in oral leukoplakia and oral squamous cell carcinoma Sci Rep, 2016-05-26;6(0):26757. 2016-05-26 [PMID: 27225603] (Bioassay, Human)
• Wang W, Zhao D, Zhou Y, Hu X, Sun Z, Yu G, Wu W, Chen S, Kuang J, Xu G, Han Z, Wang B, Yang J, Feng X Transfer of the IL-37b gene elicits anti-tumor responses in mice bearing 4T1 breast cancer. Acta Pharmacol Sin, 2015-04-01;36(4):528-34. 2015-04-01 [PMID: 25832432] (Bioassay, Mouse)

Bioinformatics

• Gene Symbol: IL37
• Uniprot:
  • Q9NZH6(Human)