Recombinant Human IL-37b/IL-1F7b Protein

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

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Catalog# & Formulation Size Price

Recombinant Human IL-37b/IL-1F7b Protein Summary

Additional Information
rh IL-37 Isoform a (Catalog # 1975-IL) is also available!
Details of Functionality
Measured by its ability to enhance IL-18 BP inhibition of IL-18-induced IFN-gamma release by KG‑1 human acute myelogenous leukemia cells. Bufler, P. et al. (2002) PNAS 99:13723. The ED50 for this effect is 2.5-12.5 μg/mL.
Source
E. coli-derived human IL-37b/IL-1F7b protein
Val46-Asp218
Accession #
N-terminal Sequence
Val46
Protein/Peptide Type
Recombinant Proteins
Gene
IL37
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
19.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
25 kDa, reducing conditions
Publications
Read Publications using
7585-IL in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS and DTT with BSA as a carrier protein.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 200 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-37b/IL-1F7b Protein

  • IL37b
  • IL-37b

Background

Human Interleukin 1 family member 7 (IL-1F7), also named FIL-1 zeta, IL-37, IL-1H4, IL-1HL and IL-1RP1, is an anti-inflammatory member of the IL-1 cytokine family. IL-1F7 shares approximately 21%, 24%, and 30% aa sequence identity with mature IL-1 alpha, IL-1 beta and IL-1ra, respectively. Mouse IL-1F7 has not been reported, but human IL-1F7 is active on mouse cells. Five isoforms (IL-1F7a through e) are generated through alternative exon usage and have distinct expression profiles (1‑5). Like IL-1 alpha, IL-1 beta and IL-18, all of the IL-1F7 variants lack a typical signal peptide. The longest IL-1F7 transcript is the basis for the product offered here. This product, also referred to as IL-1F7b, or IL-1F7 isoform 1, is normally generated from a 218 amino acid (aa) proprotein that consists of a 45 aa propeptide plus a 173 aa mature region that is cleaved by caspase-1 to generate the 32 kDa mature form (aa 46-218) (6). IL-1F7 is up-regulated by inflammatory stimuli in peripheral blood mononuclear cells (7). Experimental overexpression of IL-1F7b in vivo limits the inflammatory response and protects mice from colitis and LPS-induced shock (7, 8). Both unprocessed and mature IL-1F7b can form homodimers in solution (6, 7). Although IL-1F7b will bind to IL-18 R alpha with low affinity, this has no effect on IL‑18 receptor activity (4, 6, 9). Alternatively, IL-1F7b will also bind to IL-18 BP, generating a complex that interacts with IL-18R beta. This has the effect of attenuating IL‑18 activity via the IL-18 receptor (9). IL-1F7b can also function intracellularly. Following LPS-induced cleavage of its propeptide, IL-1F7b associates with Smad3 and translocates to the nucleus. This results in a reduction of pro-inflammatory cytokine secretion (10).
  1. Smith, D.E. et al. (2000) J. Biol Chem 275:1169.
  2. Kumar S. et al. (2000) J. Biol Chem 275:10308.
  3. Busfield S.J. et al. (2000) Genomics 66:213.
  4. Pan, G. et al. (2000) Cytokine 13:1.
  5. Boraschi, D. et al. (2011) Eur. Cytokine Netw. 22:127.
  6. Kumar S. et al. (2002) Cytokine 18:61.
  7. Nold, M.F. et al. (2010) Nat. Immunol. 11:1014.
  8. McNamee, E.N. et al. (2011) Proc. Natl. Acad. Sci. USA 108:16711.
  9. Bufler, P. et al. (2002) Proc. Natl. Acad. Sci. USA 99:13723.
  10. Sharma, S. et al. (2008) J. Immunol. 180:5477.

Publications for IL-37b/IL-1F7b (7585-IL)(4)

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Bioinformatics

Gene Symbol IL37
Uniprot