Recombinant Human IGSF9B Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human IGSF9B Protein, CF Summary

• Details of Functionality: Bioassay data are not available.
• Source: Human embryonic kidney cell, HEK293-derived human IGSF9B protein
  Glu21-Pro722, with a C-terminal 6-His tag
• Accession #: Q9UPX0
• N-terminal Sequence: Glu21
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 79 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 83-107 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IGSF9B Protein, CF

• IGSF9B
• immunoglobulin superfamily, member 9B

Background

IGSF9B, is an approximately 190 kDa transmembrane glycoprotein that plays a role in neuronal synapse maintenance and function (1). Mature human IGSF9B consists of a 702 amino acid (aa) extracellular domain (ECD) with 5 Ig-like domains and 2 fibronectin type-3 domains, a 21 aa transmembrane segment, and a 606 aa cytoplasmic domain. Within the ECD, human IGSF9B shares 97% aa sequence identity with mouse and rat IGSF9B. IGSF9B is widely expressed on interneurons in the brain where it localizes to inhibitory synapses in a ternary complex with Neuroligin-2 and S-SCAM (2). IGSF9B functions as a homophilic adhesion molecule and is required for the maturation of inhibitory synapses on interneurons (2). It shares 37% aa sequence identity with human IGSF9/Dasm1 which is expressed in the brain and dorsal root and trigeminal ganglia (3-6). Dasm1 also functions as a homophilic adhesion protein that supports the maintenance of inhibitory synapses as well as inhibitory neurotransmission (6, 7).

1. Hansen, M. and P.S. Walmod (2013) Neurochem. Res. 38:1236.
2. Woo, J. et al. (2013) J. Cell Biol. 201:929.
3. Doudney, K. et al. (2002) Genomics 79:663.
4. Shi, S.-H. et al. (2004) Proc. Natl. Acad. Sci. USA 101:13341.
5. Mishra, A. et al. (2008) Mol. Cell. Biol. 28:2782.
6. Mishra, A. et al. (2014) J. Neurosci. 34:4187.
7. Shi, S.-H. et al. (2004) Proc. Natl. Acad. Sci. USA 101:13346.

Bioinformatics

• Uniprot:
  • Q9UPX0()