Recombinant Human IgLON5 Fc Chimera Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human IgLON5 Fc Chimera Protein, CF Summary

• Details of Functionality: Bioassay data are not available.
• Source: Chinese Hamster Ovary cell line, CHO-derived human IgLON5 protein
  

  Human IGLON-5 (Leu33-Asn314) Accession # A6NGN9	IEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #: A6NGN9
• N-terminal Sequence: Leu33
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 58 kDa .
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 77-92 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IgLON5 Fc Chimera Protein, CF

• hCG1651476
• Ig-like domain-containing protein ENSP00000270642
• IgLON family member 5
• IgLON5

Background

IGLON family member 5 (IgLON5) is a member of the IgLON (LSAMP, OPCML/OBCAM, neurotrimin) family of neuronal cell adhesion molecules which, in turn, are part of the much larger Ig domain-containing superfamily (1). The overall structure of IgLON proteins contain three immunoglobulin domains, with numerous potential glycosylation sites, followed by a glycosylphosphatidylinositol (GPI) anchor (2). Mature human IgLON5 is 306 amino acids (aa) in length and shares 98% aa identity with both mouse and rat. Although the specific function of IgLON5 has yet to be elucidated, IgLON proteins are known to form homophilic and heterophilic complexes along the cell surface and several have been shown to shed from mature cortical neurons in a metalloproteinase-dependent manner. These go on to play a role in processes such as neuronal differentiation and survival, axon guidance and outgrowth and cell migration (3, 4). IgLON-5 is expressed in both neurons and oligodendrocytes (5). In the brain, the level of Iglon5 transcript is highest in the brainstem, thalamus and eye, and weakest in the hippocampus, septum, and pituitary gland (6). IgLON-5 is also expressed in non-neural organs and tissue types where it may function as a tumor-suppressor (6-7). Newly classified anti-IgLON5 syndrome is a novel neuronal tauopathy where antibodies target surface IgLON5, causing an irreversible internalization of the protein, manifesting as sleep dysfunction (8-10).

1. Sellar G, et al. (2003) Nat. Genet. 34(3):337.
2. Itoh S, et al. (2008) Biochemistry 47(38):10132.
3. Sanz R, et al. (2015) J. Biol. Chem. 290(7):4330.
4. Karagogeos D (2003) Front. Biosci. 8:1304.
5. Sharma, K. et. al. (2015) Nat. Neurosci. 18:1819.
6. Vanaveski, T. et. al. (2017) Front. Neurosci. 11:Article 38.
7. Baroy, T. et. al. (2014) Mol. Cancer 13:139.
8. Sabater L, et al. (2014) Lancet Neurol. 13(6):575.
9. Sabater L, et al. (2016) J. Neuroinflamm. 13:226.
10. Gelpi E, et al. (2016) Acta Neuropathol. 132:531.

Bioinformatics

• Uniprot:
  • A6NGN9()