Recombinant Human His6-UBE2N (Ubc13)/Uev1a Complex, CF Summary
Details of Functionality
Recombinant Human His6-UBE2N (Ubc13)/Uev1a Complex is a member of the Ubiquitin-conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin-activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-UBE2N (Ubc13)/Uev1a Complex concentration of 0.1-1 μM.
Source
E. coli-derived human UBE2N (Ubc13)/Uev1a Complex protein Contains an terminal 6-His tag
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain
Applications/Dilutions
Theoretical MW
18 kDa (UBE2N) & 21 kDa (Uev1a)
. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using E2-664 in the following applications:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -70 °C as supplied.
3 months, -70 °C under sterile conditions after opening.
Buffer
X mg/ml (X µM) in 50 mM HEPES pH 7.5, 200 mM Nacl, 10% Glycerol (v/v), 2 mM TCEP.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Recombinant Human His6-UBE2N (Ubc13)/Uev1a Complex, CF
UBE2N (Ubc13)/Uev1a Complex
Background
Ubiquitin-conjugating Enzyme E2N (UBE2N), also known as Ubiquitin-conjugating Enzyme 13 (Ubc13), forms a functional complex with the catalytically inactive Uev1a/UBE2V1 protein (1). Human UBE2N/Ubc13 shares 100% and 99% amino acid (aa) sequence identity with the mouse and rat orthologs, respectively. Human Uev1a/UBE2V1 shares 89% aa sequence identity with its mouse and rat orthologs. The UBE2N (Ubc13)/Uev1a Complex functions with Ubiquitin ligases (E3s), including TRAF-6 and TRIM5, to synthesize Lys63-linked Ubiquitin chains (2,3). These Ubiquitin chains can either be unanchored or attached to target proteins (2,4). The UBE2N (Ubc13)/Uev1a Complex localizes primarily to the cytoplasm and is important for NF-kappa B activation (5). Additionally, this complex may function in the intracellular response to retroviral capsid recognition (3). Pathologically, the UBE2N (Ubc13)/Uev1a Complex is required for the proliferation of diffuse large B-cell lymphoma cells, suggesting that it may play a role in cancer (6).
Deng, L. et al. (2000) Cell 103:351.
Xia, Z.P. et al. (2009) Nature 461:114.
Pertel, T. et al. (2011) Nature 472:361.
Liu, C. et al. (2009) Sci. Signal. 2:ra63.
Andersen, P.L. et al. (2005) J. Cell Biol. 170:745.
Pulvino, M. et al. (2012) Blood 120:1668.
Publications for UBE2N Ubc13)/Uev1a Complex (E2-664)(15)
We have publications tested in 3 confirmed species: Human, Mouse, N/A.
We have publications tested in 3 applications: Binding Assay, Bioassay, Ubiquitination.
Bioinformatics Tool for UBE2N Ubc13)/Uev1a Complex (E2-664)
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