Recombinant Human His6-UBE2N (Ubc13)/Uev1a Complex, CF

• Reactivity: Hu
• Applications: Enzyme Activity
• Format: Carrier-Free

Recombinant Human His6-UBE2N (Ubc13)/Uev1a Complex, CF Summary

• Details of Functionality: Recombinant Human His6-UBE2N (Ubc13)/Uev1a Complex is a member of the Ubiquitin-conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin-activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-UBE2N (Ubc13)/Uev1a Complex concentration of 0.1-1 μM.
• Source: E. coli-derived human UBE2N (Ubc13)/Uev1a Complex protein
  Contains an terminal 6-His tag
• Accession #: P61088 (UBE2N) & Q13404 (Uev1a)
• Protein/Peptide Type: Recombinant Enzymes
• Gene: UBE2N
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain

Applications/Dilutions

• Theoretical MW:

  18 kDa (UBE2N) & 21 kDa (Uev1a)

  .
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
• Buffer:

  X mg/ml (X µM) in 50 mM HEPES pH 7.5, 200 mM Nacl, 10% Glycerol (v/v), 2 mM TCEP.

• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human His6-UBE2N (Ubc13)/Uev1a Complex, CF

• UBE2N (Ubc13)/Uev1a Complex

Background

Ubiquitin-conjugating Enzyme E2N (UBE2N), also known as Ubiquitin-conjugating Enzyme 13 (Ubc13), forms a functional complex with the catalytically inactive Uev1a/UBE2V1 protein (1). Human UBE2N/Ubc13 shares 100% and 99% amino acid (aa) sequence identity with the mouse and rat orthologs, respectively. Human Uev1a/UBE2V1 shares 89% aa sequence identity with its mouse and rat orthologs. The UBE2N (Ubc13)/Uev1a Complex functions with Ubiquitin ligases (E3s), including TRAF-6 and TRIM5, to synthesize Lys63-linked Ubiquitin chains (2,3). These Ubiquitin chains can either be unanchored or attached to target proteins (2,4). The UBE2N (Ubc13)/Uev1a Complex localizes primarily to the cytoplasm and is important for NF-kappa B activation (5). Additionally, this complex may function in the intracellular response to retroviral capsid recognition (3). Pathologically, the UBE2N (Ubc13)/Uev1a Complex is required for the proliferation of diffuse large B-cell lymphoma cells, suggesting that it may play a role in cancer (6).

1. Deng, L. et al. (2000) Cell 103:351.
2. Xia, Z.P. et al. (2009) Nature 461:114.
3. Pertel, T. et al. (2011) Nature 472:361.
4. Liu, C. et al. (2009) Sci. Signal. 2:ra63.
5. Andersen, P.L. et al. (2005) J. Cell Biol. 170:745.
6. Pulvino, M. et al. (2012) Blood 120:1668.

Publications for UBE2N Ubc13)/Uev1a Complex (E2-664)(15)

Publications using E2-664

• CR Kim, T Noda, H Kim, G Kim, S Park, Y Na, S Oura, K Shimada, I Bang, JY Ahn, YR Kim, SK Oh, HJ Choi, JS Kim, I Jung, H Lee, Y Okada, M Ikawa, SH Baek PHF7 Modulates BRDT Stability and Histone-to-Protamine Exchange during Spermiogenesis Cell Rep, 2020;32(4):107950. 2020 [PMID: 32726616] (Bioassay, Mouse)
• M Di Rienzo, M Antonioli, C Fusco, Y Liu, M Mari, I Orhon, G Refolo, F Germani, M Corazzari, A Romagnoli, F Ciccosanti, B Mandriani, MT Pellico, R De La Torr, H Ding, M Dentice, M Neri, A Ferlini, F Reggiori, M Kulesz-Mar, M Piacentini, G Merla, GM Fimia Autophagy induction in atrophic muscle cells requires ULK1 activation by TRIM32 through unanchored K63-linked polyubiquitin chains Sci Adv, 2019;5(5):eaau8857. 2019 [PMID: 31123703] (Ubiquitination, Human)
• S Romagny, S Bouaouiche, G Lucchi, P Ducoroy, JB Bertoldo, H Terenzi, A Bettaieb, S Plenchette S-nitrosylation of cIAP1 switches cancer cell fate from TNF?/TNFR1-mediated cell survival to cell death Cancer Res., 2018;0(0):. 2018 [PMID: 29431638] (Bioassay, Human)
• C Yang, W Zang, Z Tang, Y Ji, R Xu, Y Yang, A Luo, B Hu, Z Zhang, Z Liu, X Zheng A20 regulates the DNA damage response and mediates tumor cell resistance to DNA damaging therapy Cancer Res., 2017;0(0):. 2017 [PMID: 29233925] (Bioassay, Human)
• JR Brickner, JM Soll, PM Lombardi, CB Vågbø, MC Mudge, C Oyeniran, R Rabe, J Jackson, ME Sullender, E Blazosky, AK Byrum, Y Zhao, MA Corbett, J Gécz, M Field, A Vindigni, G Slupphaug, C Wolberger, N Mosammapar A ubiquitin-dependent signalling axis specific for ALKBH-mediated DNA dealkylation repair Nature, 2017;551(7680):389-393. 2017 [PMID: 29144457] (Bioassay, Human)
• Q Ma, H Ruan, L Peng, M Zhang, MU Gack, WD Yao Proteasome-independent polyubiquitin linkage regulates synapse scaffolding, efficacy, and plasticity Proc. Natl. Acad. Sci. U.S.A., 2017;0(0):. 2017 [PMID: 28973854] (Bioassay, Human)
• K Kliza, C Taumer, I Pinzuti, M Franz-Wach, S Kunzelmann, B Stieglitz, B Macek, K Husnjak Internally tagged ubiquitin: a tool to identify linear polyubiquitin-modified proteins by mass spectrometry Nat. Methods, 2017;0(0):. 2017 [PMID: 28319114] (Bioassay, N/A)
• Xueting Lang TRIM65-catalized ubiquitination is essential for MDA5-mediated antiviral innate immunity J. Exp. Med, 2016;0(0):. 2016 [PMID: 28031478] (Bioassay, Human)
• Ekta Tripathi Cell cycle-regulated ubiquitination of tankyrase 1 by RNF8 and ABRO1/BRCC36 controls the timing of sister telomere resolution EMBO J, 2016;0(0):. 2016 [PMID: 27993934] (Bioassay)
• SKP2 loss destabilizes EZH2 by promoting TRAF6-mediated ubiquitination to suppress prostate cancer Oncogene, 2016;0(0):. 2016 [PMID: 27869166] (Bioassay)
• Hui-Kuan Lin TRAF6 Restricts p53 Mitochondrial Translocation, Apoptosis, and Tumor Suppression Mol. Cell, 2016;64(4):803-814. 2016 [PMID: 27818144] (Bioassay)
• Q Zhang, D Karnak, M Tan, TS Lawrence, MA Morgan, Y Sun FBXW7 Facilitates Nonhomologous End-Joining via K63-Linked Polyubiquitylation of XRCC4 Mol. Cell, 2016;61(3):419-33. 2016 [PMID: 26774286] (Bioassay, Human)
• Wu L, Lee S, Zhou B, Nguyen U, Muir T, Tan S, Dou Y ASH2L regulates ubiquitylation signaling to MLL: trans-regulation of H3 K4 methylation in higher eukaryotes. Mol Cell, 2013;49(6):1108-20. 2013 [PMID: 23453805]
• Smith, Christop, Berry, Donna M, McGlade, C Jane The E3 ubiquitin ligases RNF126 and Rabring7 regulate endosomal sorting of the epidermal growth factor receptor. J Cell Sci, 2013;126(0):1366-80. 2013 [PMID: 23418353] (Bioassay, N/A)
• Shibata Y, Oyama M, Kozuka-Hata H, Han X, Tanaka Y, Gohda J, Inoue J p47 negatively regulates IKK activation by inducing the lysosomal degradation of polyubiquitinated NEMO. Nat Commun, 2012;3(0):1061. 2012 [PMID: 22990857] (Binding Assay, N/A)

Bioinformatics

• Gene Symbol: UBE2N
• Uniprot:
  • P61088 (UBE2N) & Q13404 (Uev1a)()