Recombinant Human Elongin B/Elongin C/VHL/CUL2/RBX1, CF

Images

 
1 μg and 2 μg per lane of Recombinant Human VHL/ELOB/ELOC/CUL2/RBX1 Complex (Catalog # E3-655) was resolved with SDS-PAGE under reducing conditions and visualized by colloidal Coomassie Blue staining, showing bands ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Human Elongin B/Elongin C/VHL/CUL2/RBX1, CF Summary

Details of Functionality
Typical protein concentration for use in vitro will depend on experimental conditions.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human VHL/ELOB/ELOC/CUL2/RBX1 Complex protein
Accession #
Protein/Peptide Type
Recombinant Enzymes
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Applications/Dilutions

Theoretical MW
25 kDa (VHL), 13.1 kDa (ELOB), 12.8 kDa (ELOC), 93 kDa (CUL2), 12 kDa (RBX1).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 48 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Buffer
X mg/ml (X μM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% (v/v) Glycerol, 2 mM DTT
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Elongin B/Elongin C/VHL/CUL2/RBX1, CF

  • VHL/ELOB/ELOC/CUL2/RBX1 Complex

Background

VHL (von Hippel-Lindau disease tumor suppressor) is the substrate recognition subunit for an E3 ligase activity that ubiquitinates proteins containing hydroxyproline residues.  Targets of VHL include HIF1 alpha, beta 2 adrenergic receptor, ZHX2 and others.  In VHL-box E3 Ubiquitin ligases, VHL is bound to a heterodimer complex composed of Elongin B (ELOB) and Elongin C (ELOC) through the "BC-box motif" found in many proteins in the VHL-box and SOCS-box protein families.  ELOB/ELOC serves as an adapter between substrate recognition proteins and Cullin-2 (or Cullin-5) and RBX1 to form an active E3 ligase.  This protein complex contains an amino-terminal His-tag on the CUL2 subunit.
  1. Kamura T., et al. (1998) Genes & Dev.  doi: 10.1101/gad.12.24.3872
  2. Okumura F., et al. (2012) Front. Oncol.  doi: 10.3389/fonc.2012.00010
  3. Xie L., et al. (2009) Sci. Signaling  doi: 10.1126/scisignal.2000444
  4. Zhang J., et al. (2018) Science  doi: 10.1126/science.aap8411

Publications for VHL/ELOB/ELOC/CUL2/RBX1 Complex (E3-655) (0)

There are no publications for VHL/ELOB/ELOC/CUL2/RBX1 Complex (E3-655).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for VHL/ELOB/ELOC/CUL2/RBX1 Complex (E3-655) (0)

There are no reviews for VHL/ELOB/ELOC/CUL2/RBX1 Complex (E3-655). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for VHL/ELOB/ELOC/CUL2/RBX1 Complex (E3-655) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Other Available Formats

Additional VHL/ELOB/ELOC/CUL2/RBX1 Complex Products

Blogs on VHL/ELOB/ELOC/CUL2/RBX1 Complex

There are no specific blogs for VHL/ELOB/ELOC/CUL2/RBX1 Complex, but you can read our latest blog posts.

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human Elongin B/Elongin C/VHL/CUL2/RBX1, CF and receive a gift card or discount.

Bioinformatics