Recombinant Human DDB1/CRBN Complex Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human DDB1/CRBN Complex Protein, CF Summary

• Details of Functionality: Typical concentration to support in vitro applications will depend on experimental conditions.
• Source: Spodoptera frugiperda, Sf 21 (baculovirus)-derived human DDB1/CRBN Complex protein
• Accession #: Q16531.1, Q96SW2.1
• Protein/Peptide Type: Recombinant Enzymes
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 127 kDa (DDB1), 51 kDa (CRBN).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.
• Buffer: Supplied as a 0.2 μm filtered solution in HEPES, NaCl, DTT and Glycerol.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human DDB1/CRBN Complex Protein, CF

• DDB1/CRBN Complex

Background

Cereblon (CRBN) is the substrate recognition component of DCX (DDB1-CUL4-X-box) E3 Ubiquitin ligase complexes that mediate the ubiquitination of numerous target proteins including the transcriptional regulator MEIS2.  CRBN plays an important role in limb outgrowth, and its function in this process is perturbed by the binding of thalidomide and related compounds.  Binding of CRBN to Cullin-4 is mediated in part by DNA damage-binding protein 1 (DDB1), a component of multiple DCX class ligases.  In addition to its role in limb development, DDB1 is also involved in various DNA damage response mechanisms.  This product consists of an untagged complex of DDB1 and CRBN.

1. Fischer E.S. ,et al. (2014) Nature  512: 49
2. He Y.J., et al. (2006)  Genes Dev.  20: 2949
3. Ito T., et al. (2010) Science  327: 1345
4. Wang H., et al. (2006) Mol. Cell  22: 383

Publications for DDB1/CRBN Complex (E3-500)(1)

Publication using E3-500

• Meyers, M;Cismoski, S;Panidapu, A;Chie-Leon, B;Nomura, DK; Targeted Protein Degradation through Recruitment of the CUL4A Complex Adaptor Protein DDB1 bioRxiv : the preprint server for biology 2023-08-12 [PMID: 37614621] (Bioassay, N/A)

Bioinformatics

• Uniprot:
  • Q16531.1, Q96SW2.1()