Recombinant Human CUL5/RBX2 Neddylated Complex Protein, CF

• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human CUL5/RBX2 Neddylated Complex Protein, CF Summary

• Additional Information: His10.
• Details of Functionality: Typical enzyme concentration to support in vitro conjugation will depend on experimental conditions.
• Source: Spodoptera frugiperda, Sf 21 (baculovirus)-derived human CUL5/RBX2 Complex protein
  Met1 - Ala780 with a N-terminal 10-His tag (CUL5); Met1 - Lys113 (RBX2)
• Accession #: Q93034.4 (CUL5); Q9UBF6.1 (RBX2)
• Protein/Peptide Type: Recombinant Enzymes
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 102 kDa (neddylated CUL5), 13 kDa (RBX2).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
• Buffer: Supplied as a solution in HEPES, NaCl, Glycerol and DTT.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human CUL5/RBX2 Neddylated Complex Protein, CF

• CUL5/RBX2 Complex

Background

Cullin-5 (CUL5) is a core component of multiple ECS (Elongin-CUL5-SOCS-box protein) E3 ubiquitin ligase complexes that mediate the ubiquitination of proteins involved in cytokine receptor signal transduction.  In the ECS complex, CUL5 serves as a scaffold that organizes the Elongin B/Elongin C/SOCS (Suppressor of Cytokine Signaling) recognition subunits with the RBX subunit and contributes to catalysis through positioning of the substrate and an E2 ubiquitin-conjugating enzyme.  In vivo, the E3 ubiquitin ligase activity of the ECS complex is dependent on neddylation of the cullin subunit, though neddylation may be dispensable for some in vitro reactions. 

1. Baek, K., et al. (2020) Nature 578: 461.
   
2. Duda, D.M., et al. (2012) Mol. Cell 47: 371.
3. Kamura, T., et al. (2004) Genes Dev. 18: 3055.
4. Kamura, T., et al. (2001) J. Biol. Chem. 276: 29748.

Bioinformatics

• Uniprot:
  • Q93034.4 (CUL5); Q9UBF6.1 (RBX2)()